Isolation of cellulolytic enzyme producer from kenaf stem and an evaluation of kenaf bioretting process


Citation

Puvaneswary Degarajan, . and Paridah Md. Tahir, . and Wan Zuhainis Saad, . and Rosfarizan Mohamad, . (2011) Isolation of cellulolytic enzyme producer from kenaf stem and an evaluation of kenaf bioretting process. [Proceedings Paper]

Abstract

Kenaf is known as a plant species derived from the bark of Hibiscus Cannabinus L. and it has two types of fibres; the outer bark or bast portion 40 of the plant and the inner woody core material 60. In this study four strains isolated from kenaf stem were examined for their ability to produce cellulolytic enzyme that commonly used in bioretting. Bioretting is a process of separating the bast from kenaf core. In this process non-cellulosic substances are removed in order to produce textile quality bast fibres. A gram-negative strain had shown the positive result for the enzyme production. Different types of enzymes Ban Promozyme and Pectinex Ultra SP-L were also investigated and compared for their performance in bioretting process at room temperature. The enzyme retted fibres were tested for tensile strength and the average tensile strength of 262.58 MPa was obtained.


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Abstract

Kenaf is known as a plant species derived from the bark of Hibiscus Cannabinus L. and it has two types of fibres; the outer bark or bast portion 40 of the plant and the inner woody core material 60. In this study four strains isolated from kenaf stem were examined for their ability to produce cellulolytic enzyme that commonly used in bioretting. Bioretting is a process of separating the bast from kenaf core. In this process non-cellulosic substances are removed in order to produce textile quality bast fibres. A gram-negative strain had shown the positive result for the enzyme production. Different types of enzymes Ban Promozyme and Pectinex Ultra SP-L were also investigated and compared for their performance in bioretting process at room temperature. The enzyme retted fibres were tested for tensile strength and the average tensile strength of 262.58 MPa was obtained.

Additional Metadata

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Item Type: Proceedings Paper
Additional Information: Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QR22 M3I61 2011 vol.2 Call Number.
AGROVOC Term: Kenaf
AGROVOC Term: Hibiscus cannabinus
AGROVOC Term: Isolation of microorganisms
AGROVOC Term: Bark extracts
AGROVOC Term: Fibres
AGROVOC Term: Bast fibres
AGROVOC Term: Cellulolytic microorganisms
AGROVOC Term: Gram negative bacteria
AGROVOC Term: Enzymes
AGROVOC Term: Tensile strength
Geographical Term: MALAYSIA
Depositing User: Ms. Suzila Mohamad Kasim
Last Modified: 24 Apr 2025 05:15
URI: http://webagris.upm.edu.my/id/eprint/13094

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