Citation
Nurhayati Z. A., . and Law I. K., . and Noorlidah A., . (2010) Anti-human papillomavirus Anti-HPV type 16 activity of selected polyporales. [Proceedings Paper]
Abstract
Cervical cancer is the second most common cancer in women worldwide. Specific types of human papillomaviruses HPV such as HPV 16 are closely associated with the development of cervical cancer. The transforming properties of high-risk HPV 16 primarily reside in E6 gene which encodes for an oncoprotein directly involved in cellular transformation of the cervical epithelia leading to neoplasm. The E6 protein is present in cervical cancer precursor lesions and is consistently expressed in HPV-positive cervical cancer and derived cell lines. Therefore this study was undertaken to screen for natural products which suppress the expression of E6 protein. Polyporales which are Pycnoporus sanguineus KUM60501 Trametes menziesii KUM60503 and Trametes versicolor KUM611 09 were extracted from mycelia biomass using dichloromethane OCM except for T. versicolor which was also extracted using methanol. CaSki cells were incubated with each of the extracts prepared in six different concentrations and were subsequently analyzed qualitatively for anti-HPV 16 E6 activity using immunocytochemistry technique. The presence of reddish-brown product in the nuclei and/ or cytoplasm of the CaSki cells indicate the presence of HPV 16 E6 oncoprotein. The selected Polyporales extracts showed reduction in the expression of E6 protein at the different concentrations suggesting the presence of active compounds which suppress the HPV 16 E6 protein. The OCM extract of T. menziesii produced a better suppression of E6 protein at lower concentrations compared to the other extracts which showed the suppression at the higher concentration. The DCM extract of T. menziesii therefore holds a potential to be used as prevention or treatment for cervical cancer.
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Abstract
Cervical cancer is the second most common cancer in women worldwide. Specific types of human papillomaviruses HPV such as HPV 16 are closely associated with the development of cervical cancer. The transforming properties of high-risk HPV 16 primarily reside in E6 gene which encodes for an oncoprotein directly involved in cellular transformation of the cervical epithelia leading to neoplasm. The E6 protein is present in cervical cancer precursor lesions and is consistently expressed in HPV-positive cervical cancer and derived cell lines. Therefore this study was undertaken to screen for natural products which suppress the expression of E6 protein. Polyporales which are Pycnoporus sanguineus KUM60501 Trametes menziesii KUM60503 and Trametes versicolor KUM611 09 were extracted from mycelia biomass using dichloromethane OCM except for T. versicolor which was also extracted using methanol. CaSki cells were incubated with each of the extracts prepared in six different concentrations and were subsequently analyzed qualitatively for anti-HPV 16 E6 activity using immunocytochemistry technique. The presence of reddish-brown product in the nuclei and/ or cytoplasm of the CaSki cells indicate the presence of HPV 16 E6 oncoprotein. The selected Polyporales extracts showed reduction in the expression of E6 protein at the different concentrations suggesting the presence of active compounds which suppress the HPV 16 E6 protein. The OCM extract of T. menziesii produced a better suppression of E6 protein at lower concentrations compared to the other extracts which showed the suppression at the higher concentration. The DCM extract of T. menziesii therefore holds a potential to be used as prevention or treatment for cervical cancer.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. TP248.14 I61 2008 Call Number. |
| AGROVOC Term: | Polyporales |
| AGROVOC Term: | Screening tests |
| AGROVOC Term: | Ganoderma lucidum |
| AGROVOC Term: | Mushrooms |
| AGROVOC Term: | Disease treatment |
| AGROVOC Term: | Cancer disease |
| AGROVOC Term: | Papillomavirus |
| AGROVOC Term: | Protein content |
| AGROVOC Term: | Methanol |
| AGROVOC Term: | Pycnoporus |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/13152 |
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