Citation
Sabaratnam V., . and Noorlidah A., . and Nurhayati Z. A., . and Razif D., . and Lau C. C., . and Lai L. K., . (2010) Cycotoxic and anti-human papillomavirus activities of methanol and dichloromethane extracts from selected polyporales. [Proceedings Paper]
Abstract
In Malaysia cervical cancer ranks as the second most common neoplasia in women Human papillomavirus HPV type 16 is the major causative agent for cervical cancer. The E6 region of the HPV 16 genome encodes for an oncaprotein directly involved in the carcinogenesis of the disease. The E6 protein is abundant in cervical cancer and cervical cancer-derived cell lines. Realizing such impportance of the E6 oncoprotein this study was undertaken to screen for natural products which snow cytotoxic activity against cervical cancer-derived cell lines -CaSki and HeLa -and suppress the expression of HPV 16 E6 oncoprotein in CaSki. Methanol and dichloromethane crude extracts from the mycelia of selected Polyporales namely Polyporus grammacephalus KUM60500 Coriolus so KUM50012 and Trametes sp KUM50035 were prepared The cell lines were first treated with crude extracts at varying concentrations. In vitro neutral red NR cytotoxicity assay showed that only dichloromethane extract of P. grammocephalus KUM60500 showed potent activity against HeLa with an ED‚o value of 10 g/ml. ED‚o denotes the concentration of extract that inhibits the growth of 50 of the cells. Crude extracts with ED‚o values 20 g/ml are considered active. Immunocytochemistry technique was performed to analyze the expression of the HPV 16 E6 oncoprotein in CaSki cells preetreated with the Polyporales extracts. Reduction in the expression of E6 protein was observed with the best suppression activity seen in cells treated with dichloromethane extract of P. grammocephalus KUM60500. These findings suggest that P. grammacephalus KUM60500 Coriolus sp. KUM50012 and Trametes sp. KUM50035 have promising potentials to be used as therapy for cervical cancer.
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Abstract
In Malaysia cervical cancer ranks as the second most common neoplasia in women Human papillomavirus HPV type 16 is the major causative agent for cervical cancer. The E6 region of the HPV 16 genome encodes for an oncaprotein directly involved in the carcinogenesis of the disease. The E6 protein is abundant in cervical cancer and cervical cancer-derived cell lines. Realizing such impportance of the E6 oncoprotein this study was undertaken to screen for natural products which snow cytotoxic activity against cervical cancer-derived cell lines -CaSki and HeLa -and suppress the expression of HPV 16 E6 oncoprotein in CaSki. Methanol and dichloromethane crude extracts from the mycelia of selected Polyporales namely Polyporus grammacephalus KUM60500 Coriolus so KUM50012 and Trametes sp KUM50035 were prepared The cell lines were first treated with crude extracts at varying concentrations. In vitro neutral red NR cytotoxicity assay showed that only dichloromethane extract of P. grammocephalus KUM60500 showed potent activity against HeLa with an ED‚o value of 10 g/ml. ED‚o denotes the concentration of extract that inhibits the growth of 50 of the cells. Crude extracts with ED‚o values 20 g/ml are considered active. Immunocytochemistry technique was performed to analyze the expression of the HPV 16 E6 oncoprotein in CaSki cells preetreated with the Polyporales extracts. Reduction in the expression of E6 protein was observed with the best suppression activity seen in cells treated with dichloromethane extract of P. grammocephalus KUM60500. These findings suggest that P. grammacephalus KUM60500 Coriolus sp. KUM50012 and Trametes sp. KUM50035 have promising potentials to be used as therapy for cervical cancer.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. TP248.14 I61 2008 Call Number. |
| AGROVOC Term: | Polyporales |
| AGROVOC Term: | Mycelium |
| AGROVOC Term: | Microbiological analysis |
| AGROVOC Term: | Cancer disease |
| AGROVOC Term: | Papillomavirus |
| AGROVOC Term: | Extracts |
| AGROVOC Term: | Methanol |
| AGROVOC Term: | Polyporus |
| AGROVOC Term: | Coriolus |
| AGROVOC Term: | Trametes |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/13162 |
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