Citation
Mariana Nor S., . and Haslinda Ayu M. Y., . and Habsah M., . (2010) Sustainable Anti-MRSA Gracilaria changii extract through molecular biotechnology approach. [Proceedings Paper]
Abstract
Global bacterial infection management has a universal trend involving the fight against antibiotic resistant strains. The pressing microbial resistance problem incurred high infectious disease burden and cost constraints requiring application of newer more expensive agents. An approach for treatment against an antibiotic resistant pathogen MRSA involving a combination therapy is investigated. In the present study synergy was determined by the checkerboard assay. The effect in combination of commercial antibiotic and seaweed extract were screened on MRSA strain. Following the disc diffusion test the checkerboard assay giving the FIC index of 0.93 indicate that the seaweed extract works in synergy with commercial antibiotics and it has the potential as a resistance modulator. In view of the fact that there was positive effect of the extract further study were conducted molecularly on several genes to study the effect of the extract in combination with antibiotic toward the selected genes through RT PCR analysis as compared to effect of the extract alone. The synergy effect on resistant genes of MRSA namely mecA and mecl showed different nucleotide sequence changes as compared to untreated. The promising application of local G. changii as an antiMRSA agent is an added value to mega-diversity tropical flora.
Download File
Full text available from:
|
Abstract
Global bacterial infection management has a universal trend involving the fight against antibiotic resistant strains. The pressing microbial resistance problem incurred high infectious disease burden and cost constraints requiring application of newer more expensive agents. An approach for treatment against an antibiotic resistant pathogen MRSA involving a combination therapy is investigated. In the present study synergy was determined by the checkerboard assay. The effect in combination of commercial antibiotic and seaweed extract were screened on MRSA strain. Following the disc diffusion test the checkerboard assay giving the FIC index of 0.93 indicate that the seaweed extract works in synergy with commercial antibiotics and it has the potential as a resistance modulator. In view of the fact that there was positive effect of the extract further study were conducted molecularly on several genes to study the effect of the extract in combination with antibiotic toward the selected genes through RT PCR analysis as compared to effect of the extract alone. The synergy effect on resistant genes of MRSA namely mecA and mecl showed different nucleotide sequence changes as compared to untreated. The promising application of local G. changii as an antiMRSA agent is an added value to mega-diversity tropical flora.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. TP248.14 I61 2008 Call Number. |
| AGROVOC Term: | Gracilaria |
| AGROVOC Term: | Seaweeds |
| AGROVOC Term: | Extracts |
| AGROVOC Term: | Biotechnology |
| AGROVOC Term: | Molecular biology |
| AGROVOC Term: | PCR |
| AGROVOC Term: | Antibiotics |
| AGROVOC Term: | antimicrobial agents |
| AGROVOC Term: | Resistance mechanisms |
| AGROVOC Term: | Antibiotic resistance |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/13163 |
Actions (login required)
 |
View Item |
