Citation
Mejin M., . and Pyke S. M., . (2010) Isolation structural elucidation and antimicrobial activity of the chemical constituents of Scaevola spinescens. [Proceedings Paper]
Abstract
Scaevola spinescens an Australian indigenous plant has been used by the Aboriginal people in their traditional medicines for treating colds stomach ache urinary problems and pain in the alimentary tract skin rashes boils and sores. The purpose of this research is to isolate the chemical constituents of S. spinescens and to investigate their antimicrobial activity. The dried aerial parts were extracted sequentially in a Soxhlet apparatus with hexane ethyl acetate methanol and water. A water decoction was prepared as well. So far six compounds have been isolated three of which are known. The structures of these compounds were determined using 10 and 20 NMR UV-Visible spectrometry FTIR mass spectrometry and optical rotation. The structures of the remaining three isolated compounds are yet to be determined. Organic and aqueous crude extracts of Scaevola spinescens were screened for antimicrobial activity against Gram-positive Staphylococcus aureus ATCC25923 and Streptococcus pyogenes ATCC 10389 and Gram-negative Pseudomonas aeruginosa ATCC 27853 and Escherichia coli ATCC 25922 bacteria. Hexane ethyl acetate methanol and aqueous extracts were further investigated for antimicrobial activity. One of the isolated compounds has antimicrobial activity against Gram-positive bacteria with a minimum inhibitory concentration MIC ranging from 1.875 to 7.5 g/ml and minimum bactericidal concentration MBC ranging from 3.75 to 15 g/ml. No activity was observed against Gram-negative bacteria. Further investigation of the aqueous extract on the antimicrobial activity will be of interest as it may validate the remedy used by the Aboriginal people.
Download File
Full text available from:
|
Abstract
Scaevola spinescens an Australian indigenous plant has been used by the Aboriginal people in their traditional medicines for treating colds stomach ache urinary problems and pain in the alimentary tract skin rashes boils and sores. The purpose of this research is to isolate the chemical constituents of S. spinescens and to investigate their antimicrobial activity. The dried aerial parts were extracted sequentially in a Soxhlet apparatus with hexane ethyl acetate methanol and water. A water decoction was prepared as well. So far six compounds have been isolated three of which are known. The structures of these compounds were determined using 10 and 20 NMR UV-Visible spectrometry FTIR mass spectrometry and optical rotation. The structures of the remaining three isolated compounds are yet to be determined. Organic and aqueous crude extracts of Scaevola spinescens were screened for antimicrobial activity against Gram-positive Staphylococcus aureus ATCC25923 and Streptococcus pyogenes ATCC 10389 and Gram-negative Pseudomonas aeruginosa ATCC 27853 and Escherichia coli ATCC 25922 bacteria. Hexane ethyl acetate methanol and aqueous extracts were further investigated for antimicrobial activity. One of the isolated compounds has antimicrobial activity against Gram-positive bacteria with a minimum inhibitory concentration MIC ranging from 1.875 to 7.5 g/ml and minimum bactericidal concentration MBC ranging from 3.75 to 15 g/ml. No activity was observed against Gram-negative bacteria. Further investigation of the aqueous extract on the antimicrobial activity will be of interest as it may validate the remedy used by the Aboriginal people.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. TP248.14 I61 2008 Call Number. |
| AGROVOC Term: | Medicinal plants |
| AGROVOC Term: | Traditional medicines |
| AGROVOC Term: | Isolation in chemical analysis |
| AGROVOC Term: | Fractionation |
| AGROVOC Term: | Mass spectrometry |
| AGROVOC Term: | Coumarins |
| AGROVOC Term: | Terpenoids |
| AGROVOC Term: | Methanol |
| AGROVOC Term: | Iridoids |
| AGROVOC Term: | Flavonoids |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/13165 |
Actions (login required)
 |
View Item |
