Development of bio-health products from rice bran ferulates

Maznah I., . and Gururaj B., . and Ho K L., . and Nur Hazwani R., . (2010) Development of bio-health products from rice bran ferulates. [Proceedings Paper]

Rice bran the major byproduct of the rice milling industry is also the source of high quality vegetable oil viz. rice bran oil RBO. Rice bran oil has attracted much medical attention due to its strong hypocholesterolemic properties primarily attributable to its balanced fatty acid composition and high levels of antioxidant phytochemicals such as oryzanols tocopherols and tocotrienols. Rice bran and rice bran oil have particularly been under investigation recently because of their potential benefits. Rice bran oil is also rich source of phytochemicals with nutraceutical and antioxidant properties and product development could certainly add value to these bio-resources that are available in abundance. Nevertheless there is yet a study on the biological properties and identification of stabilized rice bran phytochemical rich fractions and biotransformation products from rice bran oil. The phytochemical rich fractions from RBO were successfully extracted using SFE and sequential extraction methods. Ferulate esters were isolated and purified using R-HPLC and Prep-HPLC and analyzed using LC-MS Q-TOF. Antioxidant activity of ferulate rich fractions from RBO has been determined using DPPH method and anticancer activity of ferulate derivatives from RBO has been determined using MTS assay against HT-29 cancer cell lines. The extracted ferulic acid and its derivatives campesteryl ;erulate 1 and sitosterol ferulate 2 were tested against human colon cancer HT-29 cell line using MTS cytotoxic assay. Ferulic acid derivatives campesteryl ferulate 1 and sitosterol ferulate 2 exhibited significant cytotoxic activity against the tested cancer cell line with the IIC‚o values of 420 and 465 g/ml respectively. These enriched fractions and phtytochemicals from rice bran could find application as antitumour agents and natural antioxidants for processed foods or as dietary supplements.

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Abstract

Rice bran the major byproduct of the rice milling industry is also the source of high quality vegetable oil viz. rice bran oil RBO. Rice bran oil has attracted much medical attention due to its strong hypocholesterolemic properties primarily attributable to its balanced fatty acid composition and high levels of antioxidant phytochemicals such as oryzanols tocopherols and tocotrienols. Rice bran and rice bran oil have particularly been under investigation recently because of their potential benefits. Rice bran oil is also rich source of phytochemicals with nutraceutical and antioxidant properties and product development could certainly add value to these bio-resources that are available in abundance. Nevertheless there is yet a study on the biological properties and identification of stabilized rice bran phytochemical rich fractions and biotransformation products from rice bran oil. The phytochemical rich fractions from RBO were successfully extracted using SFE and sequential extraction methods. Ferulate esters were isolated and purified using R-HPLC and Prep-HPLC and analyzed using LC-MS Q-TOF. Antioxidant activity of ferulate rich fractions from RBO has been determined using DPPH method and anticancer activity of ferulate derivatives from RBO has been determined using MTS assay against HT-29 cancer cell lines. The extracted ferulic acid and its derivatives campesteryl ;erulate 1 and sitosterol ferulate 2 were tested against human colon cancer HT-29 cell line using MTS cytotoxic assay. Ferulic acid derivatives campesteryl ferulate 1 and sitosterol ferulate 2 exhibited significant cytotoxic activity against the tested cancer cell line with the IIC‚o values of 420 and 465 g/ml respectively. These enriched fractions and phtytochemicals from rice bran could find application as antitumour agents and natural antioxidants for processed foods or as dietary supplements.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. TP248.14 I61 2008 Call Number.
AGROVOC Term:	Rice
AGROVOC Term:	Milling byproducts
AGROVOC Term:	Phytochemistry
AGROVOC Term:	Health foods
AGROVOC Term:	Antioxidants
AGROVOC Term:	Tocopherols
AGROVOC Term:	Tocotrienols
AGROVOC Term:	Cytotoxicity
AGROVOC Term:	Ferulic acid
AGROVOC Term:	Cancer disease
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:15
URI:	http://webagris.upm.edu.my/id/eprint/13174

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