Citation
Khairiah A. K., . and Mavituna F., . (2010) Production of taxol and other taxanes from selected cell lines of Taxus spp. Cell suspension cultures. [Proceedings Paper]
Abstract
In this study callus cultures from three different Taxus spp. were used as the starting materials which were induced from different tissue explants i.e.: both needles and stems of Taxus cuspidata and Taxus cuspidata nana and seedlings of Taxus baccata. Callus and cell suspension cultures were successfully initiated from the young seedlings of T. baccata The addition of 1.5 w/v insoluble polyvinylpolypyrrolidone PVPP and use of half strength of picloram in callus maintenance medium reduced the problem of cell darkening considerably. In cell suspension cultures the use of non-ionic XAD-4 adsorbent has helped to overcome the same problem. The best carbohydrate combination 15 g/L fructose 5 g/L glucose was significantly better than all other combinations of carbohydrates tested to support the growth of T. baccata cell suspension cultures. Fructose was found the best carbon source compared to sucrose and glucose. The addition of fructose 10 g/L on day8and methyl jasmonate 100 M on day 10 after incubation has increased the total Taxol and cephalomannine production to 17 and 29 mg/L respectively which was achieved from the suspension cultures initiated from needle explants of the seedlings. The presence of Taxol and cephalomannine was detected by high performance liquid chromatography HPLC.
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Abstract
In this study callus cultures from three different Taxus spp. were used as the starting materials which were induced from different tissue explants i.e.: both needles and stems of Taxus cuspidata and Taxus cuspidata nana and seedlings of Taxus baccata. Callus and cell suspension cultures were successfully initiated from the young seedlings of T. baccata The addition of 1.5 w/v insoluble polyvinylpolypyrrolidone PVPP and use of half strength of picloram in callus maintenance medium reduced the problem of cell darkening considerably. In cell suspension cultures the use of non-ionic XAD-4 adsorbent has helped to overcome the same problem. The best carbohydrate combination 15 g/L fructose 5 g/L glucose was significantly better than all other combinations of carbohydrates tested to support the growth of T. baccata cell suspension cultures. Fructose was found the best carbon source compared to sucrose and glucose. The addition of fructose 10 g/L on day8and methyl jasmonate 100 M on day 10 after incubation has increased the total Taxol and cephalomannine production to 17 and 29 mg/L respectively which was achieved from the suspension cultures initiated from needle explants of the seedlings. The presence of Taxol and cephalomannine was detected by high performance liquid chromatography HPLC.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. TP248.14 I61 2008 Call Number. |
| AGROVOC Term: | Taxus |
| AGROVOC Term: | Plant biotechnology |
| AGROVOC Term: | Cell culture |
| AGROVOC Term: | Suspension culture |
| AGROVOC Term: | Callus culture |
| AGROVOC Term: | Carbohydrates |
| AGROVOC Term: | Fructose |
| AGROVOC Term: | Isolation in chemical analysis |
| AGROVOC Term: | High performance liquid chromatography |
| AGROVOC Term: | Taxol |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/13180 |
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