Citation
Ali A. M., . and Nazri N. A., . and Ismail K. S. K., . and M.A.M. Ishak, . and Zakaria Z., . (2010) Adaptation of compact callus of pomelo Citrus grandis to liquid media as potential approach to increase growth. [Proceedings Paper]
Abstract
There were various attempts to establish suspension cultures of Citrus grandis from compact callus that proved futile. Suspension cultures provide more benefits over callus culture as cells in suspension divide faster and controllable. Tissue culture work on pomelo is aiming for ascorbic acid production independent of raw material from plant parts. An adaptation approach by reducing agar in solid media from 100 to 75 50 and 25 was used in the study. Callus grown on less percentage of agar substance for 4 weeks showed promotion to the growth and the lowest percentage of agar used 0.25 showed highest increment that is double in the fresh weight Later the treated callus showed the ability to maintain their viability in liquid medium in longer duration 11 days compared to untreated callus 7 days. To study the changes in cells structure and arrangement an investigation through scanning electron microscope SEM was carried out.
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Abstract
There were various attempts to establish suspension cultures of Citrus grandis from compact callus that proved futile. Suspension cultures provide more benefits over callus culture as cells in suspension divide faster and controllable. Tissue culture work on pomelo is aiming for ascorbic acid production independent of raw material from plant parts. An adaptation approach by reducing agar in solid media from 100 to 75 50 and 25 was used in the study. Callus grown on less percentage of agar substance for 4 weeks showed promotion to the growth and the lowest percentage of agar used 0.25 showed highest increment that is double in the fresh weight Later the treated callus showed the ability to maintain their viability in liquid medium in longer duration 11 days compared to untreated callus 7 days. To study the changes in cells structure and arrangement an investigation through scanning electron microscope SEM was carried out.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. TP248.14 I61 2008 Call Number. |
| AGROVOC Term: | Citrus grandis |
| AGROVOC Term: | Pomelo |
| AGROVOC Term: | Plant propagation |
| AGROVOC Term: | Tissue culture |
| AGROVOC Term: | Callus culture |
| AGROVOC Term: | Suspension culture |
| AGROVOC Term: | Culture media |
| AGROVOC Term: | Growth |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/13182 |
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