Citation
Ng L. T., . and Noreha M., . and Jamilah H., . and Tan L. T., . (2010) Prolyl endopeptidase PEP inhibitory activity from an endophytic fungus isolated in Sarawak. [Proceedings Paper]
Abstract
Prolyl endopeptidases PEPs are proline-specific proteases which are actively involved in every phase of metabolism in plants and animals. They have been implicated in neurological disorders such as Alzheimer;s Disease amnesia depression and schizophrenia. Hence inhibitors of PEP could play a role in the control of these diseases. Through our microbial bioprospecting programme an endophytic fungus with potential PEP inbibitory activity was isolated from yam flower in the Padawan area Kuching Sarawak. This fungus F0274 was isolated and maintained on Potato Dextrose Agar. Based on morphological examination the fungus is preliminary classified as a Fusarium sp. To study the production of PEP inhibitors the fungus was first grown statically in flasks with 20 mL Potato Dextrose Broth at 28C until mycelia covered up the surface and then shaken overnight at 200 rpm. Butanol crude extracts were prepared from the culture broths in a 1:1 ratio. PEP inhibitory assay revealed that 200 L of crude extract was able to inhibit the activity of 0.0416 Units of PEP by 90. Neither sonication of culture broth nor removal of mycelia before solvent extraction appeared to affect the inhibitory activity suggesting that the active compound is likely to be an extracellular metabolite. Optimization studies of submerge aerobic fungus culture is currently being undertaken.
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Abstract
Prolyl endopeptidases PEPs are proline-specific proteases which are actively involved in every phase of metabolism in plants and animals. They have been implicated in neurological disorders such as Alzheimer;s Disease amnesia depression and schizophrenia. Hence inhibitors of PEP could play a role in the control of these diseases. Through our microbial bioprospecting programme an endophytic fungus with potential PEP inbibitory activity was isolated from yam flower in the Padawan area Kuching Sarawak. This fungus F0274 was isolated and maintained on Potato Dextrose Agar. Based on morphological examination the fungus is preliminary classified as a Fusarium sp. To study the production of PEP inhibitors the fungus was first grown statically in flasks with 20 mL Potato Dextrose Broth at 28C until mycelia covered up the surface and then shaken overnight at 200 rpm. Butanol crude extracts were prepared from the culture broths in a 1:1 ratio. PEP inhibitory assay revealed that 200 L of crude extract was able to inhibit the activity of 0.0416 Units of PEP by 90. Neither sonication of culture broth nor removal of mycelia before solvent extraction appeared to affect the inhibitory activity suggesting that the active compound is likely to be an extracellular metabolite. Optimization studies of submerge aerobic fungus culture is currently being undertaken.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. TP248.14 I61 2008 Call Number. |
| AGROVOC Term: | Endophytes |
| AGROVOC Term: | Fungi |
| AGROVOC Term: | Isolation of microorganisms |
| AGROVOC Term: | Fusarium |
| AGROVOC Term: | Fusarium solani pisi |
| AGROVOC Term: | Extraction |
| AGROVOC Term: | Bioassays |
| AGROVOC Term: | Fermentation |
| AGROVOC Term: | Proteases |
| AGROVOC Term: | Enzyme inhibitors |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/13184 |
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