Callus induction from mature endosperm and cotyledon tissues of Jatropha curcas

Siow T. S., . and Ting S. Y. A., . and Tee C. S., . (2010) Callus induction from mature endosperm and cotyledon tissues of Jatropha curcas. [Proceedings Paper]

Jatropha curcas Euphorbiaceae is a perennial oil-bearing and multipurpose shrub native to the tropics and sub-tropics of Asia and Africa. To date the non-edible oil in Jatropha seeds is discovered to be suitable and has good value for biofuel production. However the genetic variation in seed traits was found to significantly affect the seed germination and oil yields. Therefore in vitro plant propagation provides an alternative to mass propagate the plants. In this study we aim to induce callus from mature endosperm and cotyledon explants in order to facilitate propagation studies and oil content analysis. Callus induction study was carried out using the MS basal medium supplemented with a single auxin NAA IAA IBA 2 4-D picloram or dicamba with concentrations of 0 to 8 M. Results showed that picloram dicamba and 2 4-D were more suitable auxins for callus induction using the endosperm tissues. Of these 4 M picloram gave the highest callus induction percentage 53.336.67 followed by 4 M dicamba 51.115.88 and 6 M 2 4-D 40.005.77. As for the cotyledons M 2 4-D and dicamba were suitable for callus induction with 31.11 1 0.61 and 28.894.84 of callus induction respectively. The induced calli were further maintained and multiplied on new MS basal supplemented with combination of a single cytokinin BAP kinetin TDZ or zeatin and suitable callus-inducing auxin as our preliminary observation indicated that induction medium was unsuitable. Further study to induce embryogenic callus is now being investigated for regeneration purpose and oil content analysis.

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Abstract

Jatropha curcas Euphorbiaceae is a perennial oil-bearing and multipurpose shrub native to the tropics and sub-tropics of Asia and Africa. To date the non-edible oil in Jatropha seeds is discovered to be suitable and has good value for biofuel production. However the genetic variation in seed traits was found to significantly affect the seed germination and oil yields. Therefore in vitro plant propagation provides an alternative to mass propagate the plants. In this study we aim to induce callus from mature endosperm and cotyledon explants in order to facilitate propagation studies and oil content analysis. Callus induction study was carried out using the MS basal medium supplemented with a single auxin NAA IAA IBA 2 4-D picloram or dicamba with concentrations of 0 to 8 M. Results showed that picloram dicamba and 2 4-D were more suitable auxins for callus induction using the endosperm tissues. Of these 4 M picloram gave the highest callus induction percentage 53.336.67 followed by 4 M dicamba 51.115.88 and 6 M 2 4-D 40.005.77. As for the cotyledons M 2 4-D and dicamba were suitable for callus induction with 31.11 1 0.61 and 28.894.84 of callus induction respectively. The induced calli were further maintained and multiplied on new MS basal supplemented with combination of a single cytokinin BAP kinetin TDZ or zeatin and suitable callus-inducing auxin as our preliminary observation indicated that induction medium was unsuitable. Further study to induce embryogenic callus is now being investigated for regeneration purpose and oil content analysis.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. TP248.14 I61 2008 Call Number.
AGROVOC Term:	Euphorbiaceae
AGROVOC Term:	Jatropha curcas
AGROVOC Term:	Perennial crops
AGROVOC Term:	Plant propagation
AGROVOC Term:	Tissue culture
AGROVOC Term:	Callus culture
AGROVOC Term:	Endosperm
AGROVOC Term:	Cotyledons
AGROVOC Term:	Seeds
AGROVOC Term:	Explants
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:15
URI:	http://webagris.upm.edu.my/id/eprint/13185

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