Citation
Azadeh N., . and M. A. Kadir, . and S. B. Kadzimin, . and N.A. Abdullah, . (2010) Induction of callus and protocorm-like bodies PLBs in Phalaenopsis gigantea. [Proceedings Paper]
Abstract
An efficient and reproducible method for large-scale propagation of Phalaenopsis gigantea using leaf sections has been developed. Leaf sections from young plants were cultured on New Dogashima medium NDM supplemented with cytokinins 6-Benzylaminopurine BAP thidiazuron TDZ and kinetin KIN each at 0.01 0.1 0.5 and 1.0 mgL alone and in combinations with auxins anaphthaleneacetic acid NAA at 0.01 0.1 0.5 and 1.0 mgL. The explants developed calli and protocorm-like-bodies PLBs within 8 weeks of culture. Treatment TDl in combination with auxins was found to be best for the induction of callus and PLBs.
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Abstract
An efficient and reproducible method for large-scale propagation of Phalaenopsis gigantea using leaf sections has been developed. Leaf sections from young plants were cultured on New Dogashima medium NDM supplemented with cytokinins 6-Benzylaminopurine BAP thidiazuron TDZ and kinetin KIN each at 0.01 0.1 0.5 and 1.0 mgL alone and in combinations with auxins anaphthaleneacetic acid NAA at 0.01 0.1 0.5 and 1.0 mgL. The explants developed calli and protocorm-like-bodies PLBs within 8 weeks of culture. Treatment TDl in combination with auxins was found to be best for the induction of callus and PLBs.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. TP248.14 I61 2008 Call Number. |
| AGROVOC Term: | Phalaenopsis |
| AGROVOC Term: | Endangered species |
| AGROVOC Term: | Plant propagation |
| AGROVOC Term: | Tissue culture |
| AGROVOC Term: | In vitro culture |
| AGROVOC Term: | Leaves |
| AGROVOC Term: | Auxins |
| AGROVOC Term: | Callus |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/13186 |
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