Citation
Azmi A. R., . and Maheran A. A., . and Kamaladini H., . and Haddadi F., . and Ghizan S., . (2010) Effect of TDZ and BAP on shoot proliferation from in vitro shoot tips of strawberry. [Proceedings Paper]
Abstract
An experiment was conducted to induce shoot proliferation from shoot tips derived from runner tips of strawberry cv. Camarosa. The shoot tips were cultured on Murashige and Skoog MS medium supplemented with TDZ and BAP either alone or in combination. The concentrations used were 0 to 8 M TDZ and 0 to 27 M BAP. MS medium supplemented with a moderate concentration of 4 M BAP in combination with 2 M TDZ was optimum for strawberry shoot proliferation from shoot tips derived from runner tips. In this combination of cytokinins a 100 shoot proliferation with a mean number of 14.6 shoots per explant was produced. This regeneration protocol is suitable for mass propagation of planting materials for commercial cultivation of strawberry and for the recovery of transgenic strawberry plants following genetic transformation.
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Abstract
An experiment was conducted to induce shoot proliferation from shoot tips derived from runner tips of strawberry cv. Camarosa. The shoot tips were cultured on Murashige and Skoog MS medium supplemented with TDZ and BAP either alone or in combination. The concentrations used were 0 to 8 M TDZ and 0 to 27 M BAP. MS medium supplemented with a moderate concentration of 4 M BAP in combination with 2 M TDZ was optimum for strawberry shoot proliferation from shoot tips derived from runner tips. In this combination of cytokinins a 100 shoot proliferation with a mean number of 14.6 shoots per explant was produced. This regeneration protocol is suitable for mass propagation of planting materials for commercial cultivation of strawberry and for the recovery of transgenic strawberry plants following genetic transformation.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. TP248.14 I61 2008 Call Number. |
| AGROVOC Term: | Strawberries |
| AGROVOC Term: | Plant production propagation |
| AGROVOC Term: | In vitro culture |
| AGROVOC Term: | Tissue culture |
| AGROVOC Term: | Shoot tip culture |
| AGROVOC Term: | Thidiazuron |
| AGROVOC Term: | Benzylaminopurine |
| AGROVOC Term: | Tissue proliferation |
| AGROVOC Term: | Shoots |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/13189 |
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