Immobilization of lipase enzyme from Aspergillus niger by various techniques on different support materials


Citation

Mohd Amiron E., . and Anusha N., . and Miftahu Salihin M., . and Bazli S. A., . and Norhafizi H., . and Hirzun M. Y., . and Lim C. M., . (2010) Immobilization of lipase enzyme from Aspergillus niger by various techniques on different support materials. [Proceedings Paper]

Abstract

Three support materials; Amberlite IRC-50 Silica Gel and Kielserguhr were selected among nine carriers due to the highest activity obtained. The nine carriers that has been studied were inorganic materials sea sand silica gel kielserguhr. A12O3 Zeolite inorganic salts CaCO3 CaSO4 ionexchange resins Amberlite IRC-50 and synthetic polymer polypropylene. Immobilization of lipase enzyme from Aspergillus niger was carried out by simple adsorption adsorption on ion exchange resins combined adsorption and precipitation and pure precipitation. The suitability of the support materials and techniques for the immobilization of lipase enzyme was evaluated by determining the enzyme activity protein loading immobilization efficiency and Iypolytic activity of the immobilizates before and after freeze drying. Most of the immobilizates exhibited low enzyme activity during the hydrolytic reaction. Only those prepared by ionic adsorption on Amberlite IRC-50 and simple adsorption technique on Silica Gel and Kielserguhr showed good activity after freeze drying process i.e. 35.50 U/g 21.00 U/g and 11.50 U/g respectively.


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Abstract

Three support materials; Amberlite IRC-50 Silica Gel and Kielserguhr were selected among nine carriers due to the highest activity obtained. The nine carriers that has been studied were inorganic materials sea sand silica gel kielserguhr. A12O3 Zeolite inorganic salts CaCO3 CaSO4 ionexchange resins Amberlite IRC-50 and synthetic polymer polypropylene. Immobilization of lipase enzyme from Aspergillus niger was carried out by simple adsorption adsorption on ion exchange resins combined adsorption and precipitation and pure precipitation. The suitability of the support materials and techniques for the immobilization of lipase enzyme was evaluated by determining the enzyme activity protein loading immobilization efficiency and Iypolytic activity of the immobilizates before and after freeze drying. Most of the immobilizates exhibited low enzyme activity during the hydrolytic reaction. Only those prepared by ionic adsorption on Amberlite IRC-50 and simple adsorption technique on Silica Gel and Kielserguhr showed good activity after freeze drying process i.e. 35.50 U/g 21.00 U/g and 11.50 U/g respectively.

Additional Metadata

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Item Type: Proceedings Paper
Additional Information: Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. TP248.14 I61 2008 Call Number.
AGROVOC Term: Aspergillus niger
AGROVOC Term: Immobilization
AGROVOC Term: Adsorption
AGROVOC Term: Lipases
AGROVOC Term: Enzymes
AGROVOC Term: Techniques
AGROVOC Term: Silica
AGROVOC Term: Resins
AGROVOC Term: Enzyme activity
Geographical Term: MALAYSIA
Depositing User: Ms. Suzila Mohamad Kasim
Last Modified: 24 Apr 2025 05:15
URI: http://webagris.upm.edu.my/id/eprint/13199

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