Citation
Mohd Amiron E., . and Anusha N., . and Miftahu Salihin M., . and Bazli S. A., . and Norhafizi H., . and Hirzun M. Y., . and Lim C. M., . (2010) Immobilization of lipase enzyme from Aspergillus niger by various techniques on different support materials. [Proceedings Paper]
Abstract
Three support materials; Amberlite IRC-50 Silica Gel and Kielserguhr were selected among nine carriers due to the highest activity obtained. The nine carriers that has been studied were inorganic materials sea sand silica gel kielserguhr. A12O3 Zeolite inorganic salts CaCO3 CaSO4 ionexchange resins Amberlite IRC-50 and synthetic polymer polypropylene. Immobilization of lipase enzyme from Aspergillus niger was carried out by simple adsorption adsorption on ion exchange resins combined adsorption and precipitation and pure precipitation. The suitability of the support materials and techniques for the immobilization of lipase enzyme was evaluated by determining the enzyme activity protein loading immobilization efficiency and Iypolytic activity of the immobilizates before and after freeze drying. Most of the immobilizates exhibited low enzyme activity during the hydrolytic reaction. Only those prepared by ionic adsorption on Amberlite IRC-50 and simple adsorption technique on Silica Gel and Kielserguhr showed good activity after freeze drying process i.e. 35.50 U/g 21.00 U/g and 11.50 U/g respectively.
Download File
Full text available from:
|
Abstract
Three support materials; Amberlite IRC-50 Silica Gel and Kielserguhr were selected among nine carriers due to the highest activity obtained. The nine carriers that has been studied were inorganic materials sea sand silica gel kielserguhr. A12O3 Zeolite inorganic salts CaCO3 CaSO4 ionexchange resins Amberlite IRC-50 and synthetic polymer polypropylene. Immobilization of lipase enzyme from Aspergillus niger was carried out by simple adsorption adsorption on ion exchange resins combined adsorption and precipitation and pure precipitation. The suitability of the support materials and techniques for the immobilization of lipase enzyme was evaluated by determining the enzyme activity protein loading immobilization efficiency and Iypolytic activity of the immobilizates before and after freeze drying. Most of the immobilizates exhibited low enzyme activity during the hydrolytic reaction. Only those prepared by ionic adsorption on Amberlite IRC-50 and simple adsorption technique on Silica Gel and Kielserguhr showed good activity after freeze drying process i.e. 35.50 U/g 21.00 U/g and 11.50 U/g respectively.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. TP248.14 I61 2008 Call Number. |
| AGROVOC Term: | Aspergillus niger |
| AGROVOC Term: | Immobilization |
| AGROVOC Term: | Adsorption |
| AGROVOC Term: | Lipases |
| AGROVOC Term: | Enzymes |
| AGROVOC Term: | Techniques |
| AGROVOC Term: | Silica |
| AGROVOC Term: | Resins |
| AGROVOC Term: | Enzyme activity |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/13199 |
Actions (login required)
 |
View Item |
