Molecular diagnostic tools of Fusarium oxysporum f.sp. elaeidis the causal of vascular wilt disease of oil palm

Idris A. S., . and Rusli M. H., . and Cooper R. M., . (2013) Molecular diagnostic tools of Fusarium oxysporum f.sp. elaeidis the causal of vascular wilt disease of oil palm. [Proceedings Paper]

Vascular wilt of oil palm caused by Fusarium oxysporum f. sp. elaeidis Foe is a devastating disease in West and Central Africa. As the oil palm industry in South East Asia is still expanding so is the oil palm germplasm collection through the importation of seed and pollen from Africa the centre of diversity for Elaeis guineensis. There is a risk of inadvertent spread of the disease on contaminated seed or pollen. Malaysia is the largest palm oil producer in the world and Foe remains a major threat to this industry. Therefore this study is being conducted in order to help Malaysia avoid and/or be prepared for this potential problem. Molecular diagnostic tools are being developed for rapid detection and quantification of Foe in diseased plant tissue soil seed and pollen. These tools can be used for quarantine purposes of any imported materials and to test infection of putative resistant palm genotypes. We have developed the first Foe specific primers based on virulence effector genes that excluded 70 other phylogenetically closely related Fusarium species from various hosts and origins.

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Abstract

Vascular wilt of oil palm caused by Fusarium oxysporum f. sp. elaeidis Foe is a devastating disease in West and Central Africa. As the oil palm industry in South East Asia is still expanding so is the oil palm germplasm collection through the importation of seed and pollen from Africa the centre of diversity for Elaeis guineensis. There is a risk of inadvertent spread of the disease on contaminated seed or pollen. Malaysia is the largest palm oil producer in the world and Foe remains a major threat to this industry. Therefore this study is being conducted in order to help Malaysia avoid and/or be prepared for this potential problem. Molecular diagnostic tools are being developed for rapid detection and quantification of Foe in diseased plant tissue soil seed and pollen. These tools can be used for quarantine purposes of any imported materials and to test infection of putative resistant palm genotypes. We have developed the first Foe specific primers based on virulence effector genes that excluded 70 other phylogenetically closely related Fusarium species from various hosts and origins.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. SB608 O27M939 2013 Call Number.
AGROVOC Term:	Oil palm
AGROVOC Term:	Elaeis guineensis
AGROVOC Term:	Plant disease control
AGROVOC Term:	Molecular biology
AGROVOC Term:	Polymerase chain reaction
AGROVOC Term:	PCR
AGROVOC Term:	Fusarium oxysporum
AGROVOC Term:	Vascular wilt
AGROVOC Term:	Seed
AGROVOC Term:	Pollen
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:15
URI:	http://webagris.upm.edu.my/id/eprint/13221

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