Citation
Lim Pei Hui, . and Jualang Azlan Gansau, . and Chong Khim Phin, . (2013) Potential of microorganisms isolated from forests soil to control Ganoderma spp. of oil palm. [Proceedings Paper]
Abstract
In Malaysia oil palm is suffering from one major disease Basal Stem Rot BSR caused by Ganoderma species. Sabah forests are rich in microbial diversity that might contribute to the discovery of potential microorganisms to control BSR. The objectives of this study are to investigate potential microbes from virgin forest soil against Ganoderma spp. to identify the antagonsist microbes and to investigate the compounds from the microbes which may contribute to their antimicrobial activity. Ganoderma was isolated from fresh fruiting bodies of infected oil palm and maintained on petri dishes containing Potato Dextrose Agar. Twenty soil samples were collected from selected virgin forest Crocker Range. The isolation of microbes of interest was conducted using Dilution Plate Method DPM with different types of media for different target microorganisms. There were 54 isolated fungi 72 isolated actinomycetes and 69 isolated bacteria. Dual culture assays was carried out and the percentage inhibition of radial growth PIRG against Ganoderma spp was recorded. There are 24 fungal isolates 24 actinomycetes isolates and 5 bacterial isolates showing PIRG more than 50. Microbes with high PIRG will be further identified using Biolog or PCR sequence homology. Any potential inhibitory compound will be extracted and identified from microbes showing strong PIRG against Ganoderma.
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Abstract
In Malaysia oil palm is suffering from one major disease Basal Stem Rot BSR caused by Ganoderma species. Sabah forests are rich in microbial diversity that might contribute to the discovery of potential microorganisms to control BSR. The objectives of this study are to investigate potential microbes from virgin forest soil against Ganoderma spp. to identify the antagonsist microbes and to investigate the compounds from the microbes which may contribute to their antimicrobial activity. Ganoderma was isolated from fresh fruiting bodies of infected oil palm and maintained on petri dishes containing Potato Dextrose Agar. Twenty soil samples were collected from selected virgin forest Crocker Range. The isolation of microbes of interest was conducted using Dilution Plate Method DPM with different types of media for different target microorganisms. There were 54 isolated fungi 72 isolated actinomycetes and 69 isolated bacteria. Dual culture assays was carried out and the percentage inhibition of radial growth PIRG against Ganoderma spp was recorded. There are 24 fungal isolates 24 actinomycetes isolates and 5 bacterial isolates showing PIRG more than 50. Microbes with high PIRG will be further identified using Biolog or PCR sequence homology. Any potential inhibitory compound will be extracted and identified from microbes showing strong PIRG against Ganoderma.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. SB608 O27M939 2013 Call Number. |
| AGROVOC Term: | Oil palm |
| AGROVOC Term: | Plant disease control |
| AGROVOC Term: | Biological control |
| AGROVOC Term: | Isolation of microorganisms |
| AGROVOC Term: | Ganoderma |
| AGROVOC Term: | Rots |
| AGROVOC Term: | Microorganisms |
| AGROVOC Term: | Forest soils |
| AGROVOC Term: | Pathogenic fungi |
| AGROVOC Term: | Fungi |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/13223 |
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