Molecular marker based genetic diversity analysis of Pseudomonas GanoEB1 using RAPD

Madihah A. Z., . and Idris A. S., . and Azam M. M., . (2013) Molecular marker based genetic diversity analysis of Pseudomonas GanoEB1 using RAPD. [Proceedings Paper]

The genetic diversity of endophytic bacteria Pseudomonas spp. isolated from symptomless oil palm root tissue collected in Ganoderma disease area was detected by both polymerase chain reaction PCR and random amplified polymorphic DNA-polymerase chain reaction RAPD-PCR. A few Pseudomonas strains have been recognised as potential biocontrol agents with GanoEB1 showed highly significant improvement of suppressing Ganoderma disease. Identification using 16S ribosomal DNA identified GanoEB1 as Pseudomonas aeruginosa. RAPD-PCR profile was used for building phenetic tree by unweighted pair group method with arithmetic averages UPGMA. Phenogram built up with NTSYS software showed great similarity in the topology of the tree. Results demonstrated that intraspecific genetic variation clustered the Pseudomonas isolates in the same group and believed were in same species and strain.

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Abstract

The genetic diversity of endophytic bacteria Pseudomonas spp. isolated from symptomless oil palm root tissue collected in Ganoderma disease area was detected by both polymerase chain reaction PCR and random amplified polymorphic DNA-polymerase chain reaction RAPD-PCR. A few Pseudomonas strains have been recognised as potential biocontrol agents with GanoEB1 showed highly significant improvement of suppressing Ganoderma disease. Identification using 16S ribosomal DNA identified GanoEB1 as Pseudomonas aeruginosa. RAPD-PCR profile was used for building phenetic tree by unweighted pair group method with arithmetic averages UPGMA. Phenogram built up with NTSYS software showed great similarity in the topology of the tree. Results demonstrated that intraspecific genetic variation clustered the Pseudomonas isolates in the same group and believed were in same species and strain.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. SB608 O27M939 2013 Call Number.
AGROVOC Term:	Oil palm
AGROVOC Term:	Plant disease control
AGROVOC Term:	Biological control
AGROVOC Term:	Polymerase chain reaction
AGROVOC Term:	PCR
AGROVOC Term:	Random amplified polymorphic DNA
AGROVOC Term:	Ganoderma
AGROVOC Term:	Rots
AGROVOC Term:	Pseudomonas
AGROVOC Term:	Molecular markers
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:15
URI:	http://webagris.upm.edu.my/id/eprint/13227

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