Measurement of herbicide napropamide N N-Diethyl-2-1-naphthalenyloxy propionamide using ultravioletvisible spectrophotometer

Samsuri A. W., . and F. Sadegh-Zadeh, . and Dzolkhifli O., . and B.J. Seh-Bardan, . (2009) Measurement of herbicide napropamide N N-Diethyl-2-1-naphthalenyloxy propionamide using ultravioletvisible spectrophotometer. [Proceedings Paper]

In this study we compared the measurement of napropamide using the High Pressure Liquid Chromatography HPLC with a UV-Vis spectrophotometer. An analytical grade napropamide was prepared in 0.01 M CaCI‚‚ with 200 ppm HgCI‚‚ solution to achieve a concentration range between 5 to 40 ppm of napropamide. The absorbance of napropamide was determined using both the HPLC and UV-Vis Spectrophotometer. The calibration curve for napropamide using the UV-Vis was better than the calibration curve obtained using the HPLC. A recovery study was conducted using napropamide at similar concentration range shaken in a container for 24 hr centrifuged at 7 000 rpm for 10 min and their concentrations were again measured using the HPLC and UV-Vis. This study was conducted to simulate the sorption experiment that we intended to conduct later on. Results showed that the recovery of napropamide using UV-Vis was better than the HPLC at all concentration levels. Finally we determined the effect of dissolved organic matter DOM on the measurement of napropamide in solutions. We found that even though DOM interfered with light absorption by napropamide this can be easily corrected in the dual beam UV-Vis spectrophotometer if a blank solution containing the same amount of DOM as in the sample was used. The measurement of light absorption by a napropamide plus a DOM will be directly subtracted with the absorption by the blank DOM only resulted in the measurement of absorption of light by the napropamide. High linearity of napropamide light absorption with concentrations was observed. As a conclusion UV-Vis method is a reliable method to determine the concentrations of napropamide in soil solution and it can be used as an alternative to a more sophisticated HPLC method. Our next challenge is to determine whether UV-Vis method is capable of measuring napropamide concentration from a commercial napropamide Devrinol which is manufactured with the addition of color as a safety procedure. Color will definitely absorb light and will directly interfere with the absorption of light by napropamide.

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Abstract

In this study we compared the measurement of napropamide using the High Pressure Liquid Chromatography HPLC with a UV-Vis spectrophotometer. An analytical grade napropamide was prepared in 0.01 M CaCI‚‚ with 200 ppm HgCI‚‚ solution to achieve a concentration range between 5 to 40 ppm of napropamide. The absorbance of napropamide was determined using both the HPLC and UV-Vis Spectrophotometer. The calibration curve for napropamide using the UV-Vis was better than the calibration curve obtained using the HPLC. A recovery study was conducted using napropamide at similar concentration range shaken in a container for 24 hr centrifuged at 7 000 rpm for 10 min and their concentrations were again measured using the HPLC and UV-Vis. This study was conducted to simulate the sorption experiment that we intended to conduct later on. Results showed that the recovery of napropamide using UV-Vis was better than the HPLC at all concentration levels. Finally we determined the effect of dissolved organic matter DOM on the measurement of napropamide in solutions. We found that even though DOM interfered with light absorption by napropamide this can be easily corrected in the dual beam UV-Vis spectrophotometer if a blank solution containing the same amount of DOM as in the sample was used. The measurement of light absorption by a napropamide plus a DOM will be directly subtracted with the absorption by the blank DOM only resulted in the measurement of absorption of light by the napropamide. High linearity of napropamide light absorption with concentrations was observed. As a conclusion UV-Vis method is a reliable method to determine the concentrations of napropamide in soil solution and it can be used as an alternative to a more sophisticated HPLC method. Our next challenge is to determine whether UV-Vis method is capable of measuring napropamide concentration from a commercial napropamide Devrinol which is manufactured with the addition of color as a safety procedure. Color will definitely absorb light and will directly interfere with the absorption of light by napropamide.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. S590.2 S683 2009 Call Number.
AGROVOC Term:	Herbicides
AGROVOC Term:	Napropamide
AGROVOC Term:	Determination of chemicals
AGROVOC Term:	Chemical analysis methods
AGROVOC Term:	High performance liquid chromatography
AGROVOC Term:	HPLC
AGROVOC Term:	Spectrophotometers
AGROVOC Term:	Sorption
AGROVOC Term:	Adsorption
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:16
URI:	http://webagris.upm.edu.my/id/eprint/13516

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