Isolation of a partial cDNA clone coding for Ganoderma boninense chitin deacetylase cda

Idris A. S., . and Lim F. H., . and Ahmad Parveez G. K., . and Omar A. R., . and Nor Fakhrana I., . (2011) Isolation of a partial cDNA clone coding for Ganoderma boninense chitin deacetylase cda. [Proceedings Paper]

Ganoderma is a white rot fungus that causes a major disease in oil palm. However the understanding on this plant-pathogen interaction especially at the molecular genetics level is still limited. Efforts are underway to uncover more information on the Ganoderma-oil palm interaction especially at the molecular level. In this paper we report the isolation of a partial length cDNA coding for a chitin deacetylase CDA. This enzyme modifies and protects the invading hyphae from hydrolysis by the host chitinases. The cloning was carried out via RTPCR amplification using degenerate primers which were designed based on the conserved regions of the gene. These primers were successfully used to amplify a 304 bp cDNA fragment from Ganoderma boninense. DNA sequence analysis showed the fragment has about 70 similarity to other plant pathogenic fungus cda genes. Based on this sequence gene specific primers for the amplification of 5; and 3;-end regions of the gene were synthesized. Work is underway to clone and characterize fragments amplified using the primers. The availability of the gene cDNAs could facilitate the studies to understand their functions and ultimately improve our understanding on oil palm-Ganoderma interaction.

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Abstract

Ganoderma is a white rot fungus that causes a major disease in oil palm. However the understanding on this plant-pathogen interaction especially at the molecular genetics level is still limited. Efforts are underway to uncover more information on the Ganoderma-oil palm interaction especially at the molecular level. In this paper we report the isolation of a partial length cDNA coding for a chitin deacetylase CDA. This enzyme modifies and protects the invading hyphae from hydrolysis by the host chitinases. The cloning was carried out via RTPCR amplification using degenerate primers which were designed based on the conserved regions of the gene. These primers were successfully used to amplify a 304 bp cDNA fragment from Ganoderma boninense. DNA sequence analysis showed the fragment has about 70 similarity to other plant pathogenic fungus cda genes. Based on this sequence gene specific primers for the amplification of 5; and 3;-end regions of the gene were synthesized. Work is underway to clone and characterize fragments amplified using the primers. The availability of the gene cDNAs could facilitate the studies to understand their functions and ultimately improve our understanding on oil palm-Ganoderma interaction.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. SB608 O27M939 2011 Call Number.
AGROVOC Term:	Oil palm
AGROVOC Term:	Plant disease control
AGROVOC Term:	Ganoderma
AGROVOC Term:	Pathogenic fungi
AGROVOC Term:	Isolation
AGROVOC Term:	DNA cloning
AGROVOC Term:	DNA sequence
AGROVOC Term:	Enzymes
AGROVOC Term:	Genes
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:16
URI:	http://webagris.upm.edu.my/id/eprint/13682

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