Citation
Wong H. J., . and Masahiro H., . and Muhammad Syamil A., . and Mastura M., . and Getha K., . and Hema Thopla G., . and Khor S. E., . and Lee S. S., . (2013) Discovery of a potential methicillin-resistant Staphylococcus aureus inhibitor from Ganoderma sp. FRIM550. [Proceedings Paper]
Abstract
In our screening program for useful metabolites from tropical basidiomycetes fungi associated with root diseases fungal broth extracts were prepared from isolates belonging to several species under the genera Phellinus Ganoderma Rigidoporus Tinctoporellus Lentinus and some unidentified genera. A total of 112 extracts were evaluated for antimicrobial activity against Bacillus subtilis Staphylococcus aureus and Saccharomyces cerevisiae. Differences in antimicrobial activities observed in the different genera suggested that the ability to produce bioactive compounds is not homogenously distributed among basidiomycetes. In the primary assay a total of 26 extracts exhibited IC inhibition concentration 90 against one or more test microorganisms. Out of these 7 extracts showed strong activity MIC 125 g/ml against gram-positive bacteria B. subtilis with one exhibiting strong inhibitory activity against the methicillin-resistant S. aureus MRSA. The extract was produced by cultures of fungal strain FRIM550 isolated from diseased Acacia mangium root and identified as a species of the genus Ganoderma. Chemical studies on the extract and bioactive fractions afforded an anti-MRSA compound F4009BP1 with a MIC value of 2.34 g/ml. Details of the bioactivities of this extract and compound is reported here.
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Abstract
In our screening program for useful metabolites from tropical basidiomycetes fungi associated with root diseases fungal broth extracts were prepared from isolates belonging to several species under the genera Phellinus Ganoderma Rigidoporus Tinctoporellus Lentinus and some unidentified genera. A total of 112 extracts were evaluated for antimicrobial activity against Bacillus subtilis Staphylococcus aureus and Saccharomyces cerevisiae. Differences in antimicrobial activities observed in the different genera suggested that the ability to produce bioactive compounds is not homogenously distributed among basidiomycetes. In the primary assay a total of 26 extracts exhibited IC inhibition concentration 90 against one or more test microorganisms. Out of these 7 extracts showed strong activity MIC 125 g/ml against gram-positive bacteria B. subtilis with one exhibiting strong inhibitory activity against the methicillin-resistant S. aureus MRSA. The extract was produced by cultures of fungal strain FRIM550 isolated from diseased Acacia mangium root and identified as a species of the genus Ganoderma. Chemical studies on the extract and bioactive fractions afforded an anti-MRSA compound F4009BP1 with a MIC value of 2.34 g/ml. Details of the bioactivities of this extract and compound is reported here.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QK99 M3S471 2012 Call Number |
| AGROVOC Term: | Ganoderma |
| AGROVOC Term: | Screening tests |
| AGROVOC Term: | HPLC |
| AGROVOC Term: | Secondary metabolites |
| AGROVOC Term: | Antimicrobial properties |
| AGROVOC Term: | Staphylococcus aureus |
| AGROVOC Term: | Antibacterial agents |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:16 |
| URI: | http://webagris.upm.edu.my/id/eprint/13706 |
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