Citation
Azhari H. N., . and Suria K., . and Abdurahman H. N., . (2013) Chemical compositions and cytotoxicity of alone and combination of essential oils from Ocimum basil/cum and Cymbopogon citratus. [Proceedings Paper]
Abstract
Medicinal plants play an important role as a useful resource in producing newer and safer drugs for the treatment of various diseases. Basil Ocimum basilicum and lemongrass Cymbopogon citratus are plants which have been claimed to have many traditional uses. In this study essential oils of the two plants were screened for their cytotoxicity using Brine Shimp Lethality Assay BSLA. The essential oils were extracted from fresh leaves by steam distillation; and their chemical compositions were determined by GC-MS. Citral 59.59 tran-citral 30.52 and cis-citraI 29.07 and linalool 21.60 were the predominant compounds in the C. citratus and O. basilicum respectively. Three sample solutions were prepared from the essential oils alone coded as A Iemongrass; B basil; and C i.e the synergistic combinations in ratio 1:1 of AB. All sample solutions were screened for the cytotoxicity activity. For the bioassay four different concentrations from each oil sample were prepared 10 25 50 and 100 ppm. Results obtained of the essential oils alone or in combination showed strong cytotoxic activity against brine shimp. The LC‚o values were 37.5 62.5 and 37 ppm for A Band C respectively. The essential oils showed different toxicities which could be due to the quantitative and qualitative variations in the compositions of the essential oils.
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Abstract
Medicinal plants play an important role as a useful resource in producing newer and safer drugs for the treatment of various diseases. Basil Ocimum basilicum and lemongrass Cymbopogon citratus are plants which have been claimed to have many traditional uses. In this study essential oils of the two plants were screened for their cytotoxicity using Brine Shimp Lethality Assay BSLA. The essential oils were extracted from fresh leaves by steam distillation; and their chemical compositions were determined by GC-MS. Citral 59.59 tran-citral 30.52 and cis-citraI 29.07 and linalool 21.60 were the predominant compounds in the C. citratus and O. basilicum respectively. Three sample solutions were prepared from the essential oils alone coded as A Iemongrass; B basil; and C i.e the synergistic combinations in ratio 1:1 of AB. All sample solutions were screened for the cytotoxicity activity. For the bioassay four different concentrations from each oil sample were prepared 10 25 50 and 100 ppm. Results obtained of the essential oils alone or in combination showed strong cytotoxic activity against brine shimp. The LC‚o values were 37.5 62.5 and 37 ppm for A Band C respectively. The essential oils showed different toxicities which could be due to the quantitative and qualitative variations in the compositions of the essential oils.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QK99 M3S471 2012 Call Number. |
| AGROVOC Term: | Medicinal plants |
| AGROVOC Term: | Ocimum basilicum |
| AGROVOC Term: | Cymbopogon citratus |
| AGROVOC Term: | Chemical analysis methods |
| AGROVOC Term: | Gas chromatography |
| AGROVOC Term: | Mass spectrometry |
| AGROVOC Term: | Leaf |
| AGROVOC Term: | Essential oils |
| AGROVOC Term: | Chemical composition |
| AGROVOC Term: | Cytotoxicity |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:16 |
| URI: | http://webagris.upm.edu.my/id/eprint/13715 |
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