Preliminary study on antioxidant activity and total phenolic content of Cynometra cauliflora leaves at different extraction temperatures

Mohd Hapizul U. L., . and Pin K. Y., . and Masitah M. T., . and Firmansyah J. S., . and Nurhazwani M. H., . and Noor Rasyila M. N., . and Mohd Kamal N. H., . and Rishwan Hakim K., . and Muhammad Shahrul M. N., . and Mohd Farhan A. R., . and Ameer Jasrie J., . and Zamree M. S., . (2013) Preliminary study on antioxidant activity and total phenolic content of Cynometra cauliflora leaves at different extraction temperatures. [Proceedings Paper]

Cynometra cauliflora or locally called as Nam nam is a native plant of Malaysia grown mainly in Northern Peninsular Malaysia especially in Perak. The aim of this study is to determine the effect of extraction temperature on the antioxidant activity and total phenolic content of C. cauliflora extract. Extraction of C. cauliflora leaves was carried out at different temperatures 40 60 and 80C using water prior determination of its antioxidant activity and total phenolic content. Antioxidant activity and total phenolic content were determined using 2 2-diphenyl-1-picrylhydrazyl DPPH method and Folin-Ciocalteu method respectively. Highest percentage of inhibition was detected in the C. cauliflora extract from extraction temperature of 60C 93.13 compared to others 91.20 for 40C and 88.44 for 80C using DPPH method. The same result was obtained for total phenolic concentration where the highest concentration was recorded in extract of 60C temperature 258.16 mg GAE/ 100 mg followed by 40C 257.36 mg GAE/ 100 mg and 80C 255.63 mg GAE/ 100 mg.

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Abstract

Cynometra cauliflora or locally called as Nam nam is a native plant of Malaysia grown mainly in Northern Peninsular Malaysia especially in Perak. The aim of this study is to determine the effect of extraction temperature on the antioxidant activity and total phenolic content of C. cauliflora extract. Extraction of C. cauliflora leaves was carried out at different temperatures 40 60 and 80C using water prior determination of its antioxidant activity and total phenolic content. Antioxidant activity and total phenolic content were determined using 2 2-diphenyl-1-picrylhydrazyl DPPH method and Folin-Ciocalteu method respectively. Highest percentage of inhibition was detected in the C. cauliflora extract from extraction temperature of 60C 93.13 compared to others 91.20 for 40C and 88.44 for 80C using DPPH method. The same result was obtained for total phenolic concentration where the highest concentration was recorded in extract of 60C temperature 258.16 mg GAE/ 100 mg followed by 40C 257.36 mg GAE/ 100 mg and 80C 255.63 mg GAE/ 100 mg.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QK99 M3S471 2012 Call Number.
AGROVOC Term:	Fabaceae
AGROVOC Term:	Extraction in chemical analysis
AGROVOC Term:	Leaves
AGROVOC Term:	Plant extracts
AGROVOC Term:	Temperature
AGROVOC Term:	Antioxidants
AGROVOC Term:	Phenolic content
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:16
URI:	http://webagris.upm.edu.my/id/eprint/13723

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