Citation
Tan Soon Guan, . and Hoh Boon Peng, . and Siti Shapor Siraj, . and Khatijah Yusoff, . (2003) Identification of microsatellite loci in Mystus nemurus using RAPD markers. [Proceedings Paper]
Abstract
Microsatellites have become the favourite genetic markers used in various fields since they are codominant highly polymorphic and easy to score. One major drawback of microsatellite loci analysis is that the development of these markers is laborious expensive and technically demanding. Here we report the development of six microsatellite repeats in Mystus nemurus using RAPD PCR. Specific primer pairs were designed for each of these six microsatellite repeats. These were then tested for polymorphisms. The results showed that four loci were polymorphic and a dendrogram constructed based on genetic distances obtained from the gene frequency data indicated the phylogenetic relationships among four populations of Mystus nemurus.
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Abstract
Microsatellites have become the favourite genetic markers used in various fields since they are codominant highly polymorphic and easy to score. One major drawback of microsatellite loci analysis is that the development of these markers is laborious expensive and technically demanding. Here we report the development of six microsatellite repeats in Mystus nemurus using RAPD PCR. Specific primer pairs were designed for each of these six microsatellite repeats. These were then tested for polymorphisms. The results showed that four loci were polymorphic and a dendrogram constructed based on genetic distances obtained from the gene frequency data indicated the phylogenetic relationships among four populations of Mystus nemurus.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| AGROVOC Term: | Mystus nemurus |
| AGROVOC Term: | Bagridae |
| AGROVOC Term: | Marine catfish |
| AGROVOC Term: | Biological markers organisms |
| AGROVOC Term: | Animal identification equipment |
| AGROVOC Term: | Microsatellites |
| AGROVOC Term: | RAPD |
| AGROVOC Term: | Polymorphism |
| AGROVOC Term: | Genetic variability |
| AGROVOC Term: | Animal population |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:16 |
| URI: | http://webagris.upm.edu.my/id/eprint/13821 |
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