production of Trichoderma reesei cellulase and enzymatic hydrolysis of lignocellulosic materials for chemical feedstock -production

Stener W., . and Esterbauer H., . and Hayn M., . (1984) production of Trichoderma reesei cellulase and enzymatic hydrolysis of lignocellulosic materials for chemical feedstock -production. [Proceedings Paper]

Cellulase was produced by cultivating Trichoderma reesi strains QM 9414.MCG 77 on spruce sulfite pulp in both laboratory fermentors and pilot fermentors. The culture broth contained 3-5 filter paper units/ml FPU/ml depending on the fermentation parameters. The solid enzyme prepared by solvent precipitation contained 96 protein and 0.73 FPU/mg. 0.35 units B-glucosidase/mg and 9.8 units xylanase/mg. Analytical chromatofocusing was used to further characterize the individual protein constituents of the cellulase preparations. This method appears useful for defining the enzyme quality. The T. reesi culture broth freed from the mycelia was used to saccharify 10 FPU/g 50 deg C pH 4.8 waste paper and cereal straws. Untreated materials gave only low sugar yields. Treatment of waste paper with refiners increased the sugar yield to maximal 66 of the theoretical value 66.6 g sugar/100 g dry matter. Milling was not effective in the case of wheat straw. The saccharification of this material was nearly quantitative 74 g sugars/100 g dry matter if it was pretreated by a thermal process. The paper also reports on the present status of research and development in the cellulase field in Austria the available pilot facilities and the experiences in the pilot studies

Full text available from:

Abstract

Cellulase was produced by cultivating Trichoderma reesi strains QM 9414.MCG 77 on spruce sulfite pulp in both laboratory fermentors and pilot fermentors. The culture broth contained 3-5 filter paper units/ml FPU/ml depending on the fermentation parameters. The solid enzyme prepared by solvent precipitation contained 96 protein and 0.73 FPU/mg. 0.35 units B-glucosidase/mg and 9.8 units xylanase/mg. Analytical chromatofocusing was used to further characterize the individual protein constituents of the cellulase preparations. This method appears useful for defining the enzyme quality. The T. reesi culture broth freed from the mycelia was used to saccharify 10 FPU/g 50 deg C pH 4.8 waste paper and cereal straws. Untreated materials gave only low sugar yields. Treatment of waste paper with refiners increased the sugar yield to maximal 66 of the theoretical value 66.6 g sugar/100 g dry matter. Milling was not effective in the case of wheat straw. The saccharification of this material was nearly quantitative 74 g sugars/100 g dry matter if it was pretreated by a thermal process. The paper also reports on the present status of research and development in the cellulase field in Austria the available pilot facilities and the experiences in the pilot studies

[error in script]

Item Type:	Proceedings Paper
Additional Information:	19 ref. Summary En
AGROVOC Term:	ALIMENTOS PARA ANIMALES
AGROVOC Term:	CELULASA
AGROVOC Term:	PRODUCCION
Geographical Term:	MALAYSIA
Depositing User:	Ms. Norfaezah Khomsan
Last Modified:	24 Apr 2025 05:25
URI:	http://webagris.upm.edu.my/id/eprint/14116

Actions (login required)

View Item