Xylanase and cellulase production by several molds using corn Zea mays. L. cobs as carbon substrate

Matanguihan R.M., . and San Luis M.M., . and Rosario E.J. del, . (1984) Xylanase and cellulase production by several molds using corn Zea mays. L. cobs as carbon substrate. [Proceedings Paper]

The top three xylanase producers were found to be A. niger NRRL 337 A. niger NRRL 330 and T. viride QM 9123 A. niger NRRL 337 gave the highest xylanolytic activity namely 6.26 IU/ml volumetric activity and 7.36 IU/mg protein specific activity after 48 hours of culture. Trichoderma viride QM 9123 A. niger NRRL 330 and Penicillium sp. 86 were evaluated for cellulase production using corn cobs as carbon source. The three microorganisms showed maximum filter paper volumetric activity FPVA after 61 hoursof shake flask culture. The highest activity obtained during the screening experiments was 0.119 IU/ml from Penicillium sp. 86. Production of cellulase by Penicillium sp. 86 in shake flask culture containing 2 ground corn cobs 40 mesh size gave a FPVA of 0.14 IU/ml after 72 hours of batch culture. Ground corn cobs at 2.5 soilds level showed 16 saccharification by Penicillium sp. 86 cellulase after incubation at 50 deg C pH 4.8 for six hours. Alkali-treatment enhanced saccharification to 21.4 at 2.5 solids level

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Abstract

The top three xylanase producers were found to be A. niger NRRL 337 A. niger NRRL 330 and T. viride QM 9123 A. niger NRRL 337 gave the highest xylanolytic activity namely 6.26 IU/ml volumetric activity and 7.36 IU/mg protein specific activity after 48 hours of culture. Trichoderma viride QM 9123 A. niger NRRL 330 and Penicillium sp. 86 were evaluated for cellulase production using corn cobs as carbon source. The three microorganisms showed maximum filter paper volumetric activity FPVA after 61 hoursof shake flask culture. The highest activity obtained during the screening experiments was 0.119 IU/ml from Penicillium sp. 86. Production of cellulase by Penicillium sp. 86 in shake flask culture containing 2 ground corn cobs 40 mesh size gave a FPVA of 0.14 IU/ml after 72 hours of batch culture. Ground corn cobs at 2.5 soilds level showed 16 saccharification by Penicillium sp. 86 cellulase after incubation at 50 deg C pH 4.8 for six hours. Alkali-treatment enhanced saccharification to 21.4 at 2.5 solids level

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Item Type:	Proceedings Paper
Additional Information:	6 ill.; 7 ref. Summary En
AGROVOC Term:	ZEA MAYS
AGROVOC Term:	ASPERGILLUS
AGROVOC Term:	CELULASA
AGROVOC Term:	PRODUCCION
Geographical Term:	MALAYSIA
Depositing User:	Ms. Norfaezah Khomsan
Last Modified:	24 Apr 2025 05:25
URI:	http://webagris.upm.edu.my/id/eprint/14122

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