In vitro anti-inflammatory and comparative cytotoxicity studies on methanolic extract of Enicostemma hyssopifolium

Komal, K. P. and Berlin Grace, V. M. and Hussain, S. and Siddikuzzaman, . (2023) In vitro anti-inflammatory and comparative cytotoxicity studies on methanolic extract of Enicostemma hyssopifolium. International Food Research Journal (Malaysia), 30. pp. 1285-1296. ISSN 2231 7546

Enicostemma hyssopifolium is a perineal herb widely used as an antidiabetic agent in folklore medicine. The present work was focused on the assessment of its antiinflammatory and cytotoxic properties. Its methanolic extract was evaluated for antiinflammatory activity using murine monocytic macrophage RAW 264.7 cells, and screened for its cytotoxic property in different cancer cell lines. The methanolic extract was able to potentially inhibit the bacterial lipopolysaccharides-induced inflammatory response in RAW 264.7 cells. Results of the cytotoxicity studies revealed that the methanolic extract effectively induced the cytotoxicity at considerably lower concentration in MCF-7, A-549, and COLO-205 cell lines, while the viability of HeLa, CasKi, and HT-29 cells were inhibited at comparatively higher concentrations. Results thus indicated that E. hyssopifolium possessed potent anti-inflammatory and cytotoxic properties. This necessitates further exploration of bioactive phytochemical compounds responsible for these properties for therapeutic applications.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/30%20(05)%202023/16%20-...

Abstract

Enicostemma hyssopifolium is a perineal herb widely used as an antidiabetic agent in folklore medicine. The present work was focused on the assessment of its antiinflammatory and cytotoxic properties. Its methanolic extract was evaluated for antiinflammatory activity using murine monocytic macrophage RAW 264.7 cells, and screened for its cytotoxic property in different cancer cell lines. The methanolic extract was able to potentially inhibit the bacterial lipopolysaccharides-induced inflammatory response in RAW 264.7 cells. Results of the cytotoxicity studies revealed that the methanolic extract effectively induced the cytotoxicity at considerably lower concentration in MCF-7, A-549, and COLO-205 cell lines, while the viability of HeLa, CasKi, and HT-29 cells were inhibited at comparatively higher concentrations. Results thus indicated that E. hyssopifolium possessed potent anti-inflammatory and cytotoxic properties. This necessitates further exploration of bioactive phytochemical compounds responsible for these properties for therapeutic applications.

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Item Type:	Article
AGROVOC Term:	medicinal plants
AGROVOC Term:	cytotoxicity
AGROVOC Term:	phytotherapy
AGROVOC Term:	bioactive compounds
AGROVOC Term:	statistical methods
AGROVOC Term:	methanol
AGROVOC Term:	disease control
Geographical Term:	India
Uncontrolled Keywords:	Enicostemma hyssopifolium
Depositing User:	Nor Hasnita Abdul Samat
Date Deposited:	13 Nov 2025 07:03
Last Modified:	13 Nov 2025 07:03
URI:	http://webagris.upm.edu.my/id/eprint/1430

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