Clonal propagation of coconut palm

Branton R.L., . and Blake J., . (1986) Clonal propagation of coconut palm. [Proceedings Paper]

The coconut palm was up to now propagated entirely by seed. Since this palm was generally cross-pollinated and heterozygous the resulting variation between seedlings was a serious problem. Clonal propagation from proven high-yielding disease-resistant hybrids provided uniform material for replacement planting and high quality parental stocks for breeding programmes. Clonal propagation was attempted using tissue culture techniques. Explants were obtained from mature palms of Cocos nucifera c.v. 'Malayan Dwarf' grown in Jamaica. Calloid initiated from rachillae of young inflorescences maintained on a semi-solid medium containing 2 4-D at l0-4 M. This calloid was nodular in appearance and histological investigations showed small densely cytoplasmic cells with little differentiation of cell types. Reduction of the 2 4-D concentration to about l0-8 M over several subcultures resulted in the calloid producing white embryoid-like structures. Sections through this calloid showed meristematic tissue similar to embryogenic areas in oil and date palm callus. In many cases these white embryoid-like structures developed into abnormal shoot or leaf-like structures often with a good primary root

Full text available from:

Abstract

The coconut palm was up to now propagated entirely by seed. Since this palm was generally cross-pollinated and heterozygous the resulting variation between seedlings was a serious problem. Clonal propagation from proven high-yielding disease-resistant hybrids provided uniform material for replacement planting and high quality parental stocks for breeding programmes. Clonal propagation was attempted using tissue culture techniques. Explants were obtained from mature palms of Cocos nucifera c.v. 'Malayan Dwarf' grown in Jamaica. Calloid initiated from rachillae of young inflorescences maintained on a semi-solid medium containing 2 4-D at l0-4 M. This calloid was nodular in appearance and histological investigations showed small densely cytoplasmic cells with little differentiation of cell types. Reduction of the 2 4-D concentration to about l0-8 M over several subcultures resulted in the calloid producing white embryoid-like structures. Sections through this calloid showed meristematic tissue similar to embryogenic areas in oil and date palm callus. In many cases these white embryoid-like structures developed into abnormal shoot or leaf-like structures often with a good primary root

[error in script]

Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. SB267 I61 1984 Call Number
AGROVOC Term:	Cocos nucifera
AGROVOC Term:	Plant multiplication
AGROVOC Term:	Clones
AGROVOC Term:	Tissue culture
Geographical Term:	MALAYSIA
Depositing User:	Ms. Norfaezah Khomsan
Last Modified:	24 Apr 2025 05:25
URI:	http://webagris.upm.edu.my/id/eprint/14424

Actions (login required)

View Item