Citation
Mahiran Basri, . and Abu Bakar Salleh, . and Kamaruzaman Ampon, . and Wan Md Zin Wan Yunus, . and Che Nyonya Abd Razak, . and Mohd Yusof Abd Samad, . (1990) Studies on the purification of lipase by fast protein liquid chromatography FPLC. [Proceedings Paper]
Abstract
Crude lipase was purified 11 fold with 40 percent recovery in a four-step conventional process using water extraction ammonium sulphate precipitation and fractionation through Sephadex G-25 and G-200. Using the FPLC system the crude lipase was purified in a single step using Superose 6 Mono-Q HR5/5 or Mono-P HR5/5 columns. With Superose 6 a 12-fold purification and about 90 percent recovery was obtained. With Mono-Q and Mono-P columns the recovery and purification folds were lower but in both cases two active lipase peaks were obtained.
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Abstract
Crude lipase was purified 11 fold with 40 percent recovery in a four-step conventional process using water extraction ammonium sulphate precipitation and fractionation through Sephadex G-25 and G-200. Using the FPLC system the crude lipase was purified in a single step using Superose 6 Mono-Q HR5/5 or Mono-P HR5/5 columns. With Superose 6 a 12-fold purification and about 90 percent recovery was obtained. With Mono-Q and Mono-P columns the recovery and purification folds were lower but in both cases two active lipase peaks were obtained.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | 3 graphs; 2 tables; 5 ref. Summary En |
| AGROVOC Term: | CANDIDA |
| AGROVOC Term: | TRIACILGLICEROL LIPASA |
| AGROVOC Term: | PURIFICACION/ CROMATOGRAFIA DE CAMBIO IONICO |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Norfaezah Khomsan |
| Last Modified: | 24 Apr 2025 05:26 |
| URI: | http://webagris.upm.edu.my/id/eprint/14705 |
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