Propagation and breeding of sago palm Metroxylon spp. in vitro: stimulation of embryo growth and multiple shoots from sago embryos

Hisajima S., . and Arai Y., . and Jong F.S., . and Sim E.S., . (1991) Propagation and breeding of sago palm Metroxylon spp. in vitro: stimulation of embryo growth and multiple shoots from sago embryos. [Proceedings Paper]

In vitro stimulation of embryo growth and multiple shoot induction from sago Metroxylon spp. were examined. Addition of activated charcoal and dark culture were effective from embryo germination. Stimulation of germinated embryos was examined in different conditions. Dark culture was effective for embryo growth in the initial stage and light culture in a more developed stage. No effect of gibberellic acid up to 1000 uM. vitamin B up to 25mg/L and sodium phosphate up to 510 mg was observed. Enriched Murashige and Shoog's MS medium gave better results among the three media examined. Factorial combinations of cytokinin BAP and auxin IBA NAA or 2 4-D were examined. BAP or NAA at 10 uM was effective. In three to four months some shoots could grow to around 20-25 cm in length. When in vitro-growth plantlets were planted out in potted soil all the plantlets were dead within a month. Multiple shoots or mini suckers were observed occasionally at high concentrations of plant growth regulators. Adventitious shoots also formed from cultured sago embryo. Based on these results micropropagation of sago palm may be possible through multiple shoot induction from sago ex-plants although the conditions should be improved

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Abstract

In vitro stimulation of embryo growth and multiple shoot induction from sago Metroxylon spp. were examined. Addition of activated charcoal and dark culture were effective from embryo germination. Stimulation of germinated embryos was examined in different conditions. Dark culture was effective for embryo growth in the initial stage and light culture in a more developed stage. No effect of gibberellic acid up to 1000 uM. vitamin B up to 25mg/L and sodium phosphate up to 510 mg was observed. Enriched Murashige and Shoog's MS medium gave better results among the three media examined. Factorial combinations of cytokinin BAP and auxin IBA NAA or 2 4-D were examined. BAP or NAA at 10 uM was effective. In three to four months some shoots could grow to around 20-25 cm in length. When in vitro-growth plantlets were planted out in potted soil all the plantlets were dead within a month. Multiple shoots or mini suckers were observed occasionally at high concentrations of plant growth regulators. Adventitious shoots also formed from cultured sago embryo. Based on these results micropropagation of sago palm may be possible through multiple shoot induction from sago ex-plants although the conditions should be improved

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Item Type:	Proceedings Paper
Additional Information:	6 plates; 9 tables; 26 refs.; Summary En
AGROVOC Term:	METROXYLON
AGROVOC Term:	CULTIVO DE EMBRIONES
AGROVOC Term:	PROPAGACION DE PLANTAS
AGROVOC Term:	EMBRIONES VEGETALES/ ESTIMULO
Geographical Term:	MALAYSIA
Depositing User:	Ms. Norfaezah Khomsan
Last Modified:	24 Apr 2025 05:26
URI:	http://webagris.upm.edu.my/id/eprint/14887

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