In vitro inoculation of asparagus tissue culture plantlets with vegetative hyphae of Fusarium proliferatum

Nik Norulani Nik Abd Rahman, . and Baharuddin Salleh, . (1990) In vitro inoculation of asparagus tissue culture plantlets with vegetative hyphae of Fusarium proliferatum. [Proceedings Paper]

Asparagus plantlets were inoculated in vitro at mid stem basal and shoot apex with vegetative hyphae of Fusarium proliferatum grown axenically. Epidermal layers were removed from part of the tissue except shoot apex prior to inoculation. Scanning electron micrographs showed hyphal penetration occurred between cells where the epidermal layer was absent through natural openings and stomates. No direct penetration through cells occurred where the epidermis layer is intact. Symptoms such as reddish and necrotic lesions characteristic of field infection appeared four days after inoculation

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Abstract

Asparagus plantlets were inoculated in vitro at mid stem basal and shoot apex with vegetative hyphae of Fusarium proliferatum grown axenically. Epidermal layers were removed from part of the tissue except shoot apex prior to inoculation. Scanning electron micrographs showed hyphal penetration occurred between cells where the epidermal layer was absent through natural openings and stomates. No direct penetration through cells occurred where the epidermis layer is intact. Symptoms such as reddish and necrotic lesions characteristic of field infection appeared four days after inoculation

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Item Type:	Proceedings Paper
Additional Information:	7 ills. 4 refs.; Summary En
AGROVOC Term:	ASPARAGUS
AGROVOC Term:	FUSARIUM
AGROVOC Term:	CULTIVO DE TEJIDOS
Geographical Term:	MALAYSIA
Depositing User:	Ms. Norfaezah Khomsan
Last Modified:	24 Apr 2025 05:26
URI:	http://webagris.upm.edu.my/id/eprint/14947

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