Evidence for the presence of greening disease of citrus in P. Malaysia

Lim W.H., . and Ko W.W., . and Mohd Shamsuddin O., . (1990) Evidence for the presence of greening disease of citrus in P. Malaysia. [Proceedings Paper]

Greening disease of citrus has been suspected to be present in Malaysia for sometimes based symptoms but no evidence to confirm its presence has thus far been forwarded. This paper presents the evidence based on symptomatology transmission studies and trunk injection with antibiotics. Initial graft trasmission studies done in Cameron Highlands were negative due to the low temperature there 18 approximately 5 degree celsius. Similar studies done in the warmer lowland Klang at 29 approximately 3 degree celsius showed that the disease could be transmitted to healthy sweet orange cv. Madam Vinous by double chip grafting. Positive transmission was also obtained by caging the psyllid vector Diaphorina citri with healthy Citrus seedlings. Studies to investigate disease transmission from diseased trees to healthy periwinkle Catharanthus roseus by dodder Cuscuta campestris is still in progress. Treatment of diseased trees with antibiotic oxytetracycline by trunk injection provided evidence as the decline of five of the seven treated trees was checked compared with control; in some cases recovery was 50 percent better than the control trees. Final proof was obtained when Garnier and Bove observed the greening organism GO in diseased leaf samples from Malaysia. Malaysian samples of diseased leaves with greening symptoms and insect vectors were tested against monoclonal antibodies and hybridoma supernatants 2D12 and 10A6 developed from the Indian greening organism Poona strain obtained from INRA Broudeaux France by the techniques of ELISA and immunofluorescence respectively. The results showed that the Malaysian greening organisms like those from Thailand and China did not react with the monoclonal antibodies and hybridoma supernatants indicating that the strains from those countries were distinct from the Indian Poona strain

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Abstract

Greening disease of citrus has been suspected to be present in Malaysia for sometimes based symptoms but no evidence to confirm its presence has thus far been forwarded. This paper presents the evidence based on symptomatology transmission studies and trunk injection with antibiotics. Initial graft trasmission studies done in Cameron Highlands were negative due to the low temperature there 18 approximately 5 degree celsius. Similar studies done in the warmer lowland Klang at 29 approximately 3 degree celsius showed that the disease could be transmitted to healthy sweet orange cv. Madam Vinous by double chip grafting. Positive transmission was also obtained by caging the psyllid vector Diaphorina citri with healthy Citrus seedlings. Studies to investigate disease transmission from diseased trees to healthy periwinkle Catharanthus roseus by dodder Cuscuta campestris is still in progress. Treatment of diseased trees with antibiotic oxytetracycline by trunk injection provided evidence as the decline of five of the seven treated trees was checked compared with control; in some cases recovery was 50 percent better than the control trees. Final proof was obtained when Garnier and Bove observed the greening organism GO in diseased leaf samples from Malaysia. Malaysian samples of diseased leaves with greening symptoms and insect vectors were tested against monoclonal antibodies and hybridoma supernatants 2D12 and 10A6 developed from the Indian greening organism Poona strain obtained from INRA Broudeaux France by the techniques of ELISA and immunofluorescence respectively. The results showed that the Malaysian greening organisms like those from Thailand and China did not react with the monoclonal antibodies and hybridoma supernatants indicating that the strains from those countries were distinct from the Indian Poona strain

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Item Type:	Proceedings Paper
Additional Information:	4 tables 18 refs.; Summary En
AGROVOC Term:	CITRUS
AGROVOC Term:	ENVERDECIMIENTO
AGROVOC Term:	MALASIA
Geographical Term:	MALAYSIA
Depositing User:	Ms. Norfaezah Khomsan
Last Modified:	24 Apr 2025 05:26
URI:	http://webagris.upm.edu.my/id/eprint/15029

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