Study on the toxic effect of a batch of paraffin oil overlay in mouse embryo culture in vitro

Abdullah R.B., . and Ng S.Y., . and Bakar F., . (1994) Study on the toxic effect of a batch of paraffin oil overlay in mouse embryo culture in vitro. [Proceedings Paper]

Using two methods 2 barches of paraffin oil named old and new paraffin oil were tested for toxicity before they were used as oil overlays in embryo culture studies. In the first method epididymal sperm were collected from mature male mice and were placed in microdrops of culture medium which were covered with the respective oil overlays. Using an inverted microscope observation was made to check the survival of the sperm under study. In the second method eight-cell stage mouse embryos were cultured in vitro using the respective oil overlays to be tested. A series of time-lapse photomicrographs was taken at 0 0.5 1 24 and 48 hours in culture. In the sperm motility assay the results obtained from the old batch of paraffin oil showed that the sperm became rapidly inactive and were dead within 10 minutes while those cultured in the new batch of oil survived until the next day. In the embryo culture study all the embryos cultured in the old paraffin oil started to show a pronounced blastomere swelling effect and disintegrated after half an hour in culture. At 1 h in culture the blastomeres appeared to have lysed. In contrast embryos cultured in the new batch of paraffin oil developed to the blastocyst stage. In conclusion care must taken in choosing an oil overlay during embryo culture in vitro especially the paraffin oil to ensure that it is not toxic to the embryos in culture

Full text available from:

Abstract

Using two methods 2 barches of paraffin oil named old and new paraffin oil were tested for toxicity before they were used as oil overlays in embryo culture studies. In the first method epididymal sperm were collected from mature male mice and were placed in microdrops of culture medium which were covered with the respective oil overlays. Using an inverted microscope observation was made to check the survival of the sperm under study. In the second method eight-cell stage mouse embryos were cultured in vitro using the respective oil overlays to be tested. A series of time-lapse photomicrographs was taken at 0 0.5 1 24 and 48 hours in culture. In the sperm motility assay the results obtained from the old batch of paraffin oil showed that the sperm became rapidly inactive and were dead within 10 minutes while those cultured in the new batch of oil survived until the next day. In the embryo culture study all the embryos cultured in the old paraffin oil started to show a pronounced blastomere swelling effect and disintegrated after half an hour in culture. At 1 h in culture the blastomeres appeared to have lysed. In contrast embryos cultured in the new batch of paraffin oil developed to the blastocyst stage. In conclusion care must taken in choosing an oil overlay during embryo culture in vitro especially the paraffin oil to ensure that it is not toxic to the embryos in culture

[error in script]

Item Type:	Proceedings Paper
Additional Information:	Summary En
AGROVOC Term:	RATON
AGROVOC Term:	CULTIVO DE EMBRIONES
AGROVOC Term:	SEMEN
AGROVOC Term:	SUPERVIVENCIA
AGROVOC Term:	PODER PATOGENO
AGROVOC Term:	PARAFINAS
AGROVOC Term:	TOXICIDAD
Geographical Term:	MALAYSIA
Depositing User:	Ms. Norfaezah Khomsan
Last Modified:	24 Apr 2025 05:26
URI:	http://webagris.upm.edu.my/id/eprint/15394

Actions (login required)

View Item