The carbohydrate specificity of lectin from Artocarpus integer Merr. seeds


Citation

Wan-Yaacob Ahmad, . and Zakiroh Ahmad, . (1993) The carbohydrate specificity of lectin from Artocarpus integer Merr. seeds. [Proceedings Paper]

Abstract

Lectin from Artocarpus integer Merr. seeds has been purified using rivanol 6 9-diamino-2-ethoxyacridine lactate and the yield was 1.23. The polyacrylamide gel electrophoresis PAGE gave a long band of estimated molecular weight of 103 500. Sodium dodecyl sulfate-PAGE SDS-PAGE gave three components of 24 500 30 00 and 75 800. The lectin consists of four polypeptide chains composed of two light beta- chains 24 500 and two heavy Beta-chains 30 000 of the alpha-2 Beta-2 form. Artocarpus integer lectin possesses the sugar binding site specific for alpha-D-galactosyl moiety since its hemagglutinating activity was inhibited by the simple sugar alpha-D-galactose/N-acetyl-alpha-D-galactosamine and its glycoside derivatives. Of the sugars tested the lectin showed the strongest affinity for methyl alpha-D-galactopyranoside followed by methl 3-0-alpha-D-galactopyranosyl-alpha-D-galactopyranoside


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Abstract

Lectin from Artocarpus integer Merr. seeds has been purified using rivanol 6 9-diamino-2-ethoxyacridine lactate and the yield was 1.23. The polyacrylamide gel electrophoresis PAGE gave a long band of estimated molecular weight of 103 500. Sodium dodecyl sulfate-PAGE SDS-PAGE gave three components of 24 500 30 00 and 75 800. The lectin consists of four polypeptide chains composed of two light beta- chains 24 500 and two heavy Beta-chains 30 000 of the alpha-2 Beta-2 form. Artocarpus integer lectin possesses the sugar binding site specific for alpha-D-galactosyl moiety since its hemagglutinating activity was inhibited by the simple sugar alpha-D-galactose/N-acetyl-alpha-D-galactosamine and its glycoside derivatives. Of the sugars tested the lectin showed the strongest affinity for methyl alpha-D-galactopyranoside followed by methl 3-0-alpha-D-galactopyranosyl-alpha-D-galactopyranoside

Additional Metadata

[error in script]
Item Type: Proceedings Paper
Additional Information: Summaries En Ms
AGROVOC Term: ARTOCARPUS HETEROPHYLLUS
AGROVOC Term: PURIFICACION
AGROVOC Term: ELECTROFORESIS
AGROVOC Term: LECTINA
AGROVOC Term: SEMILLA
AGROVOC Term: AZUCARES
Geographical Term: MALAYSIA
Depositing User: Ms. Norfaezah Khomsan
Last Modified: 24 Apr 2025 05:26
URI: http://webagris.upm.edu.my/id/eprint/15435

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