Biochemical polymorphism in three different strains of tilapia Oreochromis sp in Malaysia

Padmaja K., . and Selvaraj O.S., . and Mukherjee T.K., . and Tan S.G., . (1994) Biochemical polymorphism in three different strains of tilapia Oreochromis sp in Malaysia. [Proceedings Paper]

Oreochromis spp are widely recognised as one of the most important species groups for farming in a wide range of aquaculture systems from small scale was-fed fish ponds to intensive culture systems. An ACIAR supported project entitled 'Genetic Identification of Different Strains of Oreochromis tilapia Breeds and Selection Based on Progeny Testing of Broodstock for 'Improved Production' has currently been undertaken by both the University of Malaya and Queensland University of Technology Brisbane. In present study investigation was carried out to detect biochemical polymorphism in three different strains of tilapia fish namely Oreochromis niloticus Philippines strain Oreochromis niloticus Israel strain and Oreochromis mossambicus Thai strain. The fresh tissue samples of liver muscle and heart from each species were obtained for analysis. Using cellulose acetate electrophoresis ten enzymes were investigated namely Acid phosphate Alcohol dehydrogenase Fructose biphosphate Glycerol-3-phosphate dehydrogenase Glucose-6-phosphate dehydrogenase Glucose-6-phosphate isomerase Iditol dehydrogenase Lactate dehydrogenase Malate dehydrogenase and 6-Phosphogluconate dehydrogenase. Out of these systems seven were known to be polymorphic. There seems to be a high degree of genetic similarity among all the population sampled indicating that they could have been derived from a relatively recent common ancestor

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Abstract

Oreochromis spp are widely recognised as one of the most important species groups for farming in a wide range of aquaculture systems from small scale was-fed fish ponds to intensive culture systems. An ACIAR supported project entitled 'Genetic Identification of Different Strains of Oreochromis tilapia Breeds and Selection Based on Progeny Testing of Broodstock for 'Improved Production' has currently been undertaken by both the University of Malaya and Queensland University of Technology Brisbane. In present study investigation was carried out to detect biochemical polymorphism in three different strains of tilapia fish namely Oreochromis niloticus Philippines strain Oreochromis niloticus Israel strain and Oreochromis mossambicus Thai strain. The fresh tissue samples of liver muscle and heart from each species were obtained for analysis. Using cellulose acetate electrophoresis ten enzymes were investigated namely Acid phosphate Alcohol dehydrogenase Fructose biphosphate Glycerol-3-phosphate dehydrogenase Glucose-6-phosphate dehydrogenase Glucose-6-phosphate isomerase Iditol dehydrogenase Lactate dehydrogenase Malate dehydrogenase and 6-Phosphogluconate dehydrogenase. Out of these systems seven were known to be polymorphic. There seems to be a high degree of genetic similarity among all the population sampled indicating that they could have been derived from a relatively recent common ancestor

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Item Type:	Proceedings Paper
Additional Information:	Summary En
AGROVOC Term:	TILAPIA
AGROVOC Term:	ANALISIS ENZIMATICO
AGROVOC Term:	ELECTROFORESIS
AGROVOC Term:	ENZIMAS
AGROVOC Term:	POLIMORFISMO BIOQUIMICO
AGROVOC Term:	OREOCHROMIS
AGROVOC Term:	MALASIA
Geographical Term:	MALAYSIA
Depositing User:	Ms. Norfaezah Khomsan
Last Modified:	24 Apr 2025 05:26
URI:	http://webagris.upm.edu.my/id/eprint/15461

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