Optimization of enzymatic pretreatment process for enhanced biogas production from palm oil mill effluent


Citation

M. S. Tajul Islam, . and Md Zahangir Alam, . and Abdullah Al Mamun, . and Muhammad Moniruzzaman, . (2020) Optimization of enzymatic pretreatment process for enhanced biogas production from palm oil mill effluent. Journal of Energy and Environment (Malaysia), 12 (2). pp. 1-6. ISSN 1985-7462

Abstract

Anaerobic digestion (AD) of palm oil mill effluent (POME) for biogas production by four step processes such as hydrolysis, acidogenesis, acetogenesis and methanogenesis in which hydrolysis is pondered as a rate limiting step. To achieve high yield of biogas in the AD, enzymatic hydrolysis as the pretreatment method was applied to overcome the current limitation of the existing plants. Therefore, an optimization study was carried out to evaluate AD performance for biogas production. Among the parameters, three factors i.e. cellulase enzyme dose, pH and total suspended solids (TSS) were studied by the face-centered central composite design (FCCCD) under the response surface methodology (RSM) based on lipase pre-treated POME. The results showed that the maximum of 835 ml biogas was obtained with 85% COD removal from 50 m lof POME substrate after 10 days of operation while 290 ml biogas produced from control (without pretreatment). The optimum process conditions were at pH 5 and TSS of 1.5% and enzyme loading 200 CMC/ml. High coefficient of R2 value of 0.994 indicated the satisfactory fit of the model.


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Abstract

Anaerobic digestion (AD) of palm oil mill effluent (POME) for biogas production by four step processes such as hydrolysis, acidogenesis, acetogenesis and methanogenesis in which hydrolysis is pondered as a rate limiting step. To achieve high yield of biogas in the AD, enzymatic hydrolysis as the pretreatment method was applied to overcome the current limitation of the existing plants. Therefore, an optimization study was carried out to evaluate AD performance for biogas production. Among the parameters, three factors i.e. cellulase enzyme dose, pH and total suspended solids (TSS) were studied by the face-centered central composite design (FCCCD) under the response surface methodology (RSM) based on lipase pre-treated POME. The results showed that the maximum of 835 ml biogas was obtained with 85% COD removal from 50 m lof POME substrate after 10 days of operation while 290 ml biogas produced from control (without pretreatment). The optimum process conditions were at pH 5 and TSS of 1.5% and enzyme loading 200 CMC/ml. High coefficient of R2 value of 0.994 indicated the satisfactory fit of the model.

Additional Metadata

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Item Type: Article
AGROVOC Term: enzymes
AGROVOC Term: biogas
AGROVOC Term: anaerobic treatment
AGROVOC Term: lignocellulose
AGROVOC Term: sampling
AGROVOC Term: data analysis
AGROVOC Term: palm oils
AGROVOC Term: cellulase
AGROVOC Term: lipases
AGROVOC Term: sustainability
Geographical Term: Malaysia
Depositing User: Nor Hasnita Abdul Samat
Date Deposited: 20 Nov 2025 09:37
Last Modified: 20 Nov 2025 09:37
URI: http://webagris.upm.edu.my/id/eprint/1552

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