Citation
Lindayani, . and Nor Azma Yusof, . and Halijah Ibrahim, . and Norzulaani Khalid, . and M. Azmi Muda, . and Norsaadah Abdul Rahman, . (2002) Establishmet of tissue culture and antibacterial properties of selected species in the family Zingiberaceae. [Proceedings Paper]
Abstract
Crude extracts from ten species in the family Zingiberaceae were investigated for antibacterial activities. These extracts significantly inhibited the growth of Gram-positive bacteria Staphylococcus aureus strains ATCC 24213 and ATCC 29213 and Micrococcus luteus using agar diffusion assay. None of the plant extracts inhibited the growth of the Gram-negative bacterium Escherichia coli strains ATCC 25922 and ATCC 35213. The in vitro propagation study of these species through tissue culture technique was successfully carried out. Three to five shoots per explant were successfully regenerated on MS medium supplemented with 3 sucrose 0.2 phytagel and 1.0 to 3.0 mg/ml BAP. Early studies of Zingiber zerumbet Smith and Z. ottensii showed that the rhizome yield from in vitro plantlets were higher than those from in vivo materials. Tissue culture material could be adopted for propagation purposes.
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Abstract
Crude extracts from ten species in the family Zingiberaceae were investigated for antibacterial activities. These extracts significantly inhibited the growth of Gram-positive bacteria Staphylococcus aureus strains ATCC 24213 and ATCC 29213 and Micrococcus luteus using agar diffusion assay. None of the plant extracts inhibited the growth of the Gram-negative bacterium Escherichia coli strains ATCC 25922 and ATCC 35213. The in vitro propagation study of these species through tissue culture technique was successfully carried out. Three to five shoots per explant were successfully regenerated on MS medium supplemented with 3 sucrose 0.2 phytagel and 1.0 to 3.0 mg/ml BAP. Early studies of Zingiber zerumbet Smith and Z. ottensii showed that the rhizome yield from in vitro plantlets were higher than those from in vivo materials. Tissue culture material could be adopted for propagation purposes.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. mal QK99 A1S471 2001 Call Number. |
| AGROVOC Term: | ZINGIBERACEAE |
| AGROVOC Term: | IN VITRO |
| AGROVOC Term: | EXPLANTS |
| AGROVOC Term: | PLANT EXTRACTS |
| AGROVOC Term: | TISSUE CULTURE |
| AGROVOC Term: | MALAYSIA |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Norfaezah Khomsan |
| Last Modified: | 24 Apr 2025 05:26 |
| URI: | http://webagris.upm.edu.my/id/eprint/15627 |
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