Application of solid-phase microextraction in cocoa volatiles analysis

Suzannah Sharif, . and Aznie Aida Ahmad, . (2006) Application of solid-phase microextraction in cocoa volatiles analysis. [Proceedings Paper]

Solid phase micro extraction SPME is a sample preparation technique using a fused-silica fiber that is coated on the outside with an appropriate stationary phase. Analytes which can be in the headspace or in liquid is directly extracted to the fiber coating. The SPME technique can be used routinely in combination with gas chromatography GC GC-mass spectrometry GC-MS high performance liquid chromatography HPLC or LC-MS. This technique had been used in environmental analysis aroma and flavor analysis of foods. For the analysis of cocoa volatiles in roasted nib and cocoa powder the optimum condition was 15 mins stabilization at 60C and extraction for 15 mins. Comparisons of three type of fibers were done and the best fiber was the PDMS/DVB 65mum fiber. The volatiles compounds identified consist of 3-methyl butanal pentanal methyl pyrazine 2 5-dimethyl pyrazine 2 6 dimethylpyrazine ethyl pyrazine benzaldehyde limonene linalool benzeneethanol trimethylpyrazine tetramethylpyrazine benzeneacetaldehyde nonanal and 5-methyl-2-hexenal. All the compounds had been previously reported in cocoa volatiles.

Full text available from:

Abstract

Solid phase micro extraction SPME is a sample preparation technique using a fused-silica fiber that is coated on the outside with an appropriate stationary phase. Analytes which can be in the headspace or in liquid is directly extracted to the fiber coating. The SPME technique can be used routinely in combination with gas chromatography GC GC-mass spectrometry GC-MS high performance liquid chromatography HPLC or LC-MS. This technique had been used in environmental analysis aroma and flavor analysis of foods. For the analysis of cocoa volatiles in roasted nib and cocoa powder the optimum condition was 15 mins stabilization at 60C and extraction for 15 mins. Comparisons of three type of fibers were done and the best fiber was the PDMS/DVB 65mum fiber. The volatiles compounds identified consist of 3-methyl butanal pentanal methyl pyrazine 2 5-dimethyl pyrazine 2 6 dimethylpyrazine ethyl pyrazine benzaldehyde limonene linalool benzeneethanol trimethylpyrazine tetramethylpyrazine benzeneacetaldehyde nonanal and 5-methyl-2-hexenal. All the compounds had been previously reported in cocoa volatiles.

[error in script]

Item Type:	Proceedings Paper
Additional Information:	6ill. 1 ref.
AGROVOC Term:	THEOBROMA CACAO
AGROVOC Term:	FLAVOUR
AGROVOC Term:	COCOA BEANS
AGROVOC Term:	COCOA POWDER
AGROVOC Term:	EXTRACTION
AGROVOC Term:	VOLATILE COMPOUNDS
AGROVOC Term:	ANALYSIS
AGROVOC Term:	MALAYSIA
Geographical Term:	MALAYSIA
Depositing User:	Ms. Norfaezah Khomsan
Last Modified:	24 Apr 2025 05:27
URI:	http://webagris.upm.edu.my/id/eprint/15667

Actions (login required)

View Item