Development of simple sequence of simple sequence repeat markers for oil palm and their application in genetic mapping and fingerprinting of tissue culture clones


Citation

Rajinder Singh, . and Jayanthi Nagappan, . and Rahimah Abdul Rahman, . and Tan S.G., . and Jothi Malar Panandam, . and Cheah S.C., . (2007) Development of simple sequence of simple sequence repeat markers for oil palm and their application in genetic mapping and fingerprinting of tissue culture clones. [Proceedings Paper]

Abstract

This study describes the application of a simple and effective method to isolate SSR markers from oil palm genomic sequences. A total of 12 informative SSR markers are described. The SSR markers were found suitable for genome analysis and DNA fingerprinting of oil palm tissue culture clones. Eleven of the 12 SSR markers exhibited expected Mendelian segregation ratios when tested on a mapping population indicating their suitability for genetic mapping studies. The markers identified in the species Elaeis oleifera also showed applicability in a second species that is Elaeis guineensis. Apart from genetic mapping the SSR markers also showed promise as molecular probes for DNA fingerprinting of oil palm tissue culture clones. The SSR markers can be used for clonal identification and detecting culture mix-up.


Download File

Full text available from:

Abstract

This study describes the application of a simple and effective method to isolate SSR markers from oil palm genomic sequences. A total of 12 informative SSR markers are described. The SSR markers were found suitable for genome analysis and DNA fingerprinting of oil palm tissue culture clones. Eleven of the 12 SSR markers exhibited expected Mendelian segregation ratios when tested on a mapping population indicating their suitability for genetic mapping studies. The markers identified in the species Elaeis oleifera also showed applicability in a second species that is Elaeis guineensis. Apart from genetic mapping the SSR markers also showed promise as molecular probes for DNA fingerprinting of oil palm tissue culture clones. The SSR markers can be used for clonal identification and detecting culture mix-up.

Additional Metadata

[error in script]
Item Type: Proceedings Paper
Additional Information: Summary En
AGROVOC Term: ELAEIS GUINEENSIS
AGROVOC Term: ELAEIS OLEIFERA
AGROVOC Term: TISSUE CULTURE
AGROVOC Term: CLONES
AGROVOC Term: GENOMICS
AGROVOC Term: PCR
AGROVOC Term: EXTRACTION
AGROVOC Term: GENETIC MAPS
AGROVOC Term: MALAYSIA
Geographical Term: MALAYSIA
Depositing User: Ms. Norfaezah Khomsan
Last Modified: 24 Apr 2025 05:27
URI: http://webagris.upm.edu.my/id/eprint/15716

Actions (login required)

View Item View Item