Two dimensional electrophoresis application for Ganoderma study in oil palm

Syahanim Shahwan, . (2007) Two dimensional electrophoresis application for Ganoderma study in oil palm. [Proceedings Paper]

Crop plants have become a major focus world wide today due to their ability to retain the economical status of the country. However the major problem facing the crop industry is the infection by various pathogens such as fungus. Root and stem rots caused by Ganoderma have been known to cause extensive losses of many crops. And now the fungus threats the oil palm industry. Study was conducted to develop method for extracting total proteins in the oil palm root. Total protein was extracted using various methods. As a preliminary study protein was extracted based on three different methods: Giavalisco et al. 2003 M1 Modified method by Giavaliso et al. 2003 M2 and Sheffield et al. 2006 M3. The M1 protocol showed good quality spots with reduced background for primary roots. However this method was applied to the secondary roots and heavy vertical streaking was observed at the acidic part of the strip. M2 was conducted to reduce this problem however the vertical streaking was still seen at the acidic segment. Therefore M3 was conducted and vertical streaking problem successfully reduced. Extracted protein was resolved using broad range pH 3-10 IPG strips in the first dimension. Then second dimension was conducted using 10 SDS-P AG E gels. Gels were stained with Silver Staining Kit Bio-Rad Hercules CA and the spots will be analyzed using PO-Quest Software Bio-Rad Hercules CA.

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Abstract

Crop plants have become a major focus world wide today due to their ability to retain the economical status of the country. However the major problem facing the crop industry is the infection by various pathogens such as fungus. Root and stem rots caused by Ganoderma have been known to cause extensive losses of many crops. And now the fungus threats the oil palm industry. Study was conducted to develop method for extracting total proteins in the oil palm root. Total protein was extracted using various methods. As a preliminary study protein was extracted based on three different methods: Giavalisco et al. 2003 M1 Modified method by Giavaliso et al. 2003 M2 and Sheffield et al. 2006 M3. The M1 protocol showed good quality spots with reduced background for primary roots. However this method was applied to the secondary roots and heavy vertical streaking was observed at the acidic part of the strip. M2 was conducted to reduce this problem however the vertical streaking was still seen at the acidic segment. Therefore M3 was conducted and vertical streaking problem successfully reduced. Extracted protein was resolved using broad range pH 3-10 IPG strips in the first dimension. Then second dimension was conducted using 10 SDS-P AG E gels. Gels were stained with Silver Staining Kit Bio-Rad Hercules CA and the spots will be analyzed using PO-Quest Software Bio-Rad Hercules CA.

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Item Type:	Proceedings Paper
Additional Information:	Summary En
AGROVOC Term:	ELAEIS GUINEENSIS
AGROVOC Term:	ELECTROPHORESIS
AGROVOC Term:	GANODERMA
AGROVOC Term:	INFECTION
AGROVOC Term:	PATHOGENS
AGROVOC Term:	PROTEINS
AGROVOC Term:	EXTRACTION
AGROVOC Term:	PLANT DISEASES
AGROVOC Term:	DISEASE CONTROL
AGROVOC Term:	MALAYSIA
Geographical Term:	MALAYSIA
Depositing User:	Ms. Norfaezah Khomsan
Last Modified:	24 Apr 2025 05:27
URI:	http://webagris.upm.edu.my/id/eprint/15717

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