Biotransformation of palm oil into sophorolipid biosurfactant and the application of MALDI-TOF MS

Abdul Rashid Yatim, . (2007) Biotransformation of palm oil into sophorolipid biosurfactant and the application of MALDI-TOF MS. [Proceedings Paper]

Biosurfactants are emerging surfactants produced by many single cell micro-organisms. Biosurfactants have several advantages over chemical surfactants such as lower toxicity higher biodegradability higher specificity and effectiveness at extreme temperatures or pH values. Sophorolipid consists of biosurfactant molecules produced extracellularly into the medium by the yeast C. bombicola. Sophorolipid contains the dimeric sugar sophorose 1-2-glucose linked to the hydrophobic long chain carboxylic acid with hydroxyl functionality on the penultimate or terminal carbon. In the production of sophorolipid biosurfactant we observed a significant increased in the yield when a second carbon source is available to the yeast. This secondary carbon source is contributed by the addition of palm olein at specific rate and stages of the bioprocess. Palm-based sophorolipid biosurfactant production is a two-stage bioprocess where sophorolipid is mainly produced after a first stage of growth ending because of nitrogen limitation. MALDI-TOF mass spectrometric technique was applied in the monitoring of the fermentation process. The broth and the bioproducts were directly analysed with minimum treatment. We were able to detect rapidly with a total turn around time of two minutes per analysis and accurately monitor the complex lipidic bioproducts. Furthermore the multi-component biosurfactants were also detected accurately with MALDI-TOF MS.

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Abstract

Biosurfactants are emerging surfactants produced by many single cell micro-organisms. Biosurfactants have several advantages over chemical surfactants such as lower toxicity higher biodegradability higher specificity and effectiveness at extreme temperatures or pH values. Sophorolipid consists of biosurfactant molecules produced extracellularly into the medium by the yeast C. bombicola. Sophorolipid contains the dimeric sugar sophorose 1-2-glucose linked to the hydrophobic long chain carboxylic acid with hydroxyl functionality on the penultimate or terminal carbon. In the production of sophorolipid biosurfactant we observed a significant increased in the yield when a second carbon source is available to the yeast. This secondary carbon source is contributed by the addition of palm olein at specific rate and stages of the bioprocess. Palm-based sophorolipid biosurfactant production is a two-stage bioprocess where sophorolipid is mainly produced after a first stage of growth ending because of nitrogen limitation. MALDI-TOF mass spectrometric technique was applied in the monitoring of the fermentation process. The broth and the bioproducts were directly analysed with minimum treatment. We were able to detect rapidly with a total turn around time of two minutes per analysis and accurately monitor the complex lipidic bioproducts. Furthermore the multi-component biosurfactants were also detected accurately with MALDI-TOF MS.

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Item Type:	Proceedings Paper
AGROVOC Term:	PALM OILS
AGROVOC Term:	SURFACTANTS
AGROVOC Term:	YEASTS
AGROVOC Term:	HYDROPHOBICITY
AGROVOC Term:	ORGANIC ACIDS
AGROVOC Term:	MASS SPECTROMETRY
AGROVOC Term:	FERMENTATION
AGROVOC Term:	MALAYSIA
Geographical Term:	MALAYSIA
Depositing User:	Ms. Norfaezah Khomsan
Last Modified:	24 Apr 2025 05:27
URI:	http://webagris.upm.edu.my/id/eprint/15723

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