Citation
Fairuz Ridzlan A. Rashid, . and A. R. Bahaman, . and S. Khairani-Bejo, . and A. R. Mutalib, . (2009) Detection of pathogenic leptospiral isolates from water and soil samples. [Proceedings Paper]
Abstract
Leptospirosis is an important zoonotic disease of worldwide distribution. It is an infectious bacterial disease caused by Leptospira interrogans. Rats are the major natural reservoir for these bacteria Bahaman et al. 1987. Leptospires can survive for a long period of time in the environment under favorable condition. The two modes of transmission are through direct or indirect contact: Interaction between the maintenance host contaminated environment with pathogenic leptospires and the susceptible animal plays a role in the transmission of leptospirosis. Currently about twenty- nine pathogenic leptospires serovars have been isolated from natural waters and wet soils in Malaysia Alexander et aI. 1975. Lester and LeFebvre 2003 suggested that Polymerase Chain Reaction PCR is a reliable tool for detecting leptospiral DNA from environment and clinical samples. The study conducted at a National Service Training Centre PLKN Kelantan aimed to detect the presence of pathogenic leptospires in the environment. The ability for pathogenic leptospires to survive for long period of time may possibly lead to an outbreak of the disease in that area. Seventy-four water and soil samples were collected from the camp area. The temperature and pH of the sample were recorded. The samples were inoculated into semisolid JS medium and incubated at room temperature for 3 to 12 weeks. Cultures were examined under dark-field microscope for positive growth of leptospires. Polymerase Chain Reaction was performed using G1/G2 primers for confirmation of pathogenic leptospires. Positive growth was seen in ten out of the 74 samples but only seven isolates were confirmed to be pathogenic Figure 1. Ponds and streams serve as drinking water a getaway method from extensive heat and discharge urine or faeces of animals Diesch et al. 1969. The findings of this study are similar to Luchini et al. 2008 in which they found L. interrogans in environmental samples including water from two different toilets soil from a pond rat faeces and rat bladder. They concluded that the infection to humans through contaminated environment is classified as an occupational accident.
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Abstract
Leptospirosis is an important zoonotic disease of worldwide distribution. It is an infectious bacterial disease caused by Leptospira interrogans. Rats are the major natural reservoir for these bacteria Bahaman et al. 1987. Leptospires can survive for a long period of time in the environment under favorable condition. The two modes of transmission are through direct or indirect contact: Interaction between the maintenance host contaminated environment with pathogenic leptospires and the susceptible animal plays a role in the transmission of leptospirosis. Currently about twenty- nine pathogenic leptospires serovars have been isolated from natural waters and wet soils in Malaysia Alexander et aI. 1975. Lester and LeFebvre 2003 suggested that Polymerase Chain Reaction PCR is a reliable tool for detecting leptospiral DNA from environment and clinical samples. The study conducted at a National Service Training Centre PLKN Kelantan aimed to detect the presence of pathogenic leptospires in the environment. The ability for pathogenic leptospires to survive for long period of time may possibly lead to an outbreak of the disease in that area. Seventy-four water and soil samples were collected from the camp area. The temperature and pH of the sample were recorded. The samples were inoculated into semisolid JS medium and incubated at room temperature for 3 to 12 weeks. Cultures were examined under dark-field microscope for positive growth of leptospires. Polymerase Chain Reaction was performed using G1/G2 primers for confirmation of pathogenic leptospires. Positive growth was seen in ten out of the 74 samples but only seven isolates were confirmed to be pathogenic Figure 1. Ponds and streams serve as drinking water a getaway method from extensive heat and discharge urine or faeces of animals Diesch et al. 1969. The findings of this study are similar to Luchini et al. 2008 in which they found L. interrogans in environmental samples including water from two different toilets soil from a pond rat faeces and rat bladder. They concluded that the infection to humans through contaminated environment is classified as an occupational accident.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | 1 ill. 5 ref. |
| AGROVOC Term: | Pathogenic viruses |
| AGROVOC Term: | Leptospira |
| AGROVOC Term: | Soil |
| AGROVOC Term: | Leptospirosis |
| AGROVOC Term: | Zoonoses |
| AGROVOC Term: | Water |
| AGROVOC Term: | Polymerase chain reaction |
| AGROVOC Term: | Malaysia |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Norfaezah Khomsan |
| Last Modified: | 24 Apr 2025 05:27 |
| URI: | http://webagris.upm.edu.my/id/eprint/15776 |
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