Loop-mediated isothermal amplification Lamp as a diagnostic tool for Jembrana disease virus

Asmarani Kusumawati, . and Sri Hartati, . and Puji Astuti, . and Tri Untari, . and Narendra Yoga Hendarta, . (2009) Loop-mediated isothermal amplification Lamp as a diagnostic tool for Jembrana disease virus. [Proceedings Paper]

Jembrana disease virus IJDV is the agent of an acute infectious Jembrana disease of Bali cattle Boss javanicutes reported for the first time in Jembrana village in Bali Indonesia. Jembrana disease is not unique to Boss javanicus other types of cattle are also susceptible although the resulting lesion is milder. The aim of the research was to develop molecular diagnostic tool using loop-mediated isothemal amplification LAMP. A set of primer specific to env-tm gene composing of outer primers F3 B3 inner primers FIP BIP and loop primers IF IB designed from sequence data genebank env-tm. Finally due to its ease of use LAMP is an efficient and specific diagnostic tool of Jembrana disease and an ideal method in conditions where sophisticated equipment are not available.

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Abstract

Jembrana disease virus IJDV is the agent of an acute infectious Jembrana disease of Bali cattle Boss javanicutes reported for the first time in Jembrana village in Bali Indonesia. Jembrana disease is not unique to Boss javanicus other types of cattle are also susceptible although the resulting lesion is milder. The aim of the research was to develop molecular diagnostic tool using loop-mediated isothemal amplification LAMP. A set of primer specific to env-tm gene composing of outer primers F3 B3 inner primers FIP BIP and loop primers IF IB designed from sequence data genebank env-tm. Finally due to its ease of use LAMP is an efficient and specific diagnostic tool of Jembrana disease and an ideal method in conditions where sophisticated equipment are not available.

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Item Type:	Proceedings Paper
AGROVOC Term:	Acute disease
AGROVOC Term:	Cattle
AGROVOC Term:	Genes
Geographical Term:	MALAYSIA
Depositing User:	Ms. Norfaezah Khomsan
Last Modified:	24 Apr 2025 05:27
URI:	http://webagris.upm.edu.my/id/eprint/15778

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