Ratio of thyroxine and triiodothyronine to long road transportation of Bligon buck

Pudji Astuti, . and Sarmin, . and Claude Mona Airin, . and Asmarani Kusumawati, . and Hera Maheshwari, . and Luthfiralda Sjahfirdi, . (2009) Ratio of thyroxine and triiodothyronine to long road transportation of Bligon buck. [Proceedings Paper]

Circulating thyroid hormone can be considered as indicators ofthe metabolic and nutritional status of the animals while metabolism crowding noise handling isolation agitation and extreme temperature are physical stimuli which may subject to transported animals. The aim of this study was to determine the ratio of plasma T4 and T3 concentration during animals transportation. Six adult Bligon buck with body weight ranging from 26-30 Kg were used in this study. All animals were fed standard diet in their pen at 10 of their body weight per head daily and commercial concentrate also given everyday. Fresh water was provided ad libitum. All animals were transported around village for 16 hours starting from 18.00 pm until 10.00 am in open small truck 3 x 2 m; eye contact each others would be possible. Blood samples were withdrawn from jugular vein using vacutainer tubes containing heparin then centrifuged at 500 g for 15 minutes. The blood were collected every 4 hours from 8 hours before transportation at 10.00 am 14.00 pm and 18.00 pm until the time of arriving at 10.00 am. Plasma was harvested and stored at -20 C until T3 and T4 concentrations were measured using ELISA method. The result showed that transportation of Bligon bucks for 16 hours have an affect on ratio of T4 and T3 . This ratio fluctuated significantly with time of collection P0.05 except between -8 and -4 and 4-8 after animal loading. During transportation decreasing of T4 levels indicated conversion of T4. to T3 to form active hormone.

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Abstract

Circulating thyroid hormone can be considered as indicators ofthe metabolic and nutritional status of the animals while metabolism crowding noise handling isolation agitation and extreme temperature are physical stimuli which may subject to transported animals. The aim of this study was to determine the ratio of plasma T4 and T3 concentration during animals transportation. Six adult Bligon buck with body weight ranging from 26-30 Kg were used in this study. All animals were fed standard diet in their pen at 10 of their body weight per head daily and commercial concentrate also given everyday. Fresh water was provided ad libitum. All animals were transported around village for 16 hours starting from 18.00 pm until 10.00 am in open small truck 3 x 2 m; eye contact each others would be possible. Blood samples were withdrawn from jugular vein using vacutainer tubes containing heparin then centrifuged at 500 g for 15 minutes. The blood were collected every 4 hours from 8 hours before transportation at 10.00 am 14.00 pm and 18.00 pm until the time of arriving at 10.00 am. Plasma was harvested and stored at -20 C until T3 and T4 concentrations were measured using ELISA method. The result showed that transportation of Bligon bucks for 16 hours have an affect on ratio of T4 and T3 . This ratio fluctuated significantly with time of collection P0.05 except between -8 and -4 and 4-8 after animal loading. During transportation decreasing of T4 levels indicated conversion of T4. to T3 to form active hormone.

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Item Type:	Proceedings Paper
Additional Information:	1 tables
AGROVOC Term:	Thyroxine
AGROVOC Term:	Triiodothyronine
AGROVOC Term:	Transportation
AGROVOC Term:	Thyroid hormones
AGROVOC Term:	Goats
Geographical Term:	MALAYSIA
Depositing User:	Ms. Norfaezah Khomsan
Last Modified:	24 Apr 2025 05:27
URI:	http://webagris.upm.edu.my/id/eprint/15788

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