Bacterial Restriction Endonuclease DNA Analysis Brenda for the identification of leptospiral isolates

Eileen S. T., . and Bahaman A. R., . and Mutalib A. R., . and Khairani-Bejo S., . (2009) Bacterial Restriction Endonuclease DNA Analysis Brenda for the identification of leptospiral isolates. [Proceedings Paper]

Leptospirosis is a zoonosis of worldwide distribution caused by the pathogenic species of the genus Leptospira. Leptospiral infection is transmitted via direct or indirect contact with the urine ofan infected animal. Both pathogenic and saprophytic leptospires can survive in the environment such as ponds rivers and wet soil for a long period of time. Microscopic Agglutination Test MAT has been the standard test for the serological identification of leptospires. Great interest was given to molecular typing due to the limitations associated with serological characterization. Various genotyping techniques have been introduced in identifying leptospiral isolates. However the Bacterial Restriction Endonuclease DNA Analysis BRENDA is highly recommended as it is inexpensive and less laborious. This technique has been studied extensively and was shown to give stable reproducible and distinctive fingerprints. In this study restriction endonucleases frequent cullers were used to digest the chromosomal DNA of the isolates. The fragments were then separated by agarose gel electrophoresis generating characteristic DNA fingerprints. The DNA banding patterns were compared to those of the reference strains. Reproducible characteristic bands from each isolate could be clearly observed in fragments larger than 3.5kb. In conclusion BRENDA is a simple reproducible and economical genotyping method which is useful in the epidemiological investigations of Leptospirosis outbreaks.

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Abstract

Leptospirosis is a zoonosis of worldwide distribution caused by the pathogenic species of the genus Leptospira. Leptospiral infection is transmitted via direct or indirect contact with the urine ofan infected animal. Both pathogenic and saprophytic leptospires can survive in the environment such as ponds rivers and wet soil for a long period of time. Microscopic Agglutination Test MAT has been the standard test for the serological identification of leptospires. Great interest was given to molecular typing due to the limitations associated with serological characterization. Various genotyping techniques have been introduced in identifying leptospiral isolates. However the Bacterial Restriction Endonuclease DNA Analysis BRENDA is highly recommended as it is inexpensive and less laborious. This technique has been studied extensively and was shown to give stable reproducible and distinctive fingerprints. In this study restriction endonucleases frequent cullers were used to digest the chromosomal DNA of the isolates. The fragments were then separated by agarose gel electrophoresis generating characteristic DNA fingerprints. The DNA banding patterns were compared to those of the reference strains. Reproducible characteristic bands from each isolate could be clearly observed in fragments larger than 3.5kb. In conclusion BRENDA is a simple reproducible and economical genotyping method which is useful in the epidemiological investigations of Leptospirosis outbreaks.

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Item Type:	Proceedings Paper
Additional Information:	6 ref.
AGROVOC Term:	Leptospirosis
AGROVOC Term:	DNA
AGROVOC Term:	DNA fingerprinting
AGROVOC Term:	Leptospira
AGROVOC Term:	Microscopy
AGROVOC Term:	Microscopy”Technique
Geographical Term:	MALAYSIA
Depositing User:	Ms. Norfaezah Khomsan
Last Modified:	24 Apr 2025 05:27
URI:	http://webagris.upm.edu.my/id/eprint/15822

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