Isolation of Escherichia coli 0157:H7 from cattle using immunomagnetic separation and molecular techniques

Nor Hanini S., . and Zunita Z., . and Goh Y. M., . and Awis Q. S., . (2009) Isolation of Escherichia coli 0157:H7 from cattle using immunomagnetic separation and molecular techniques. [Proceedings Paper]

Escherichia coli 0157:H7 is a well known Gram negative food borne pathogen from the pathotype of enterhemorrhagic EHEC. It has been recognized as the cause of hemorrhagic colitis HC which eventually can become severe and develop to another dangerous illness and somewhat prevalent among diarrhea pediatric patients which is hemolytic uremic syndrome HUS. The pathogenesis of E. coli 0157:H7 infections is usually related to the ability of this bacterium to colonize on the mucosal cell and excrete its toxins and cause disease but interestingly this bacteria may colonize ruminants' gut especially cattle and cause no harm to them. The bacteria is able to survive in the human food chain due to cross contamination which is why it is a concern in public health and also a threat in veterinary field as well as food industry due to its spreading in foods. In this study 30 cattle in the Shah Alam abattoir were sampled and subjected to Immunomagnetic separation lMS technique for the isolation of E. coli 0 157:H7 to increase the sensitivity to detect the bacteria. A total of two positive samples were found. The isolates were confirmed using Polymerase Chain Reaction PCR with three specific genes comprising of the somatic 'O' and flagella 'H' antigens.

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Abstract

Escherichia coli 0157:H7 is a well known Gram negative food borne pathogen from the pathotype of enterhemorrhagic EHEC. It has been recognized as the cause of hemorrhagic colitis HC which eventually can become severe and develop to another dangerous illness and somewhat prevalent among diarrhea pediatric patients which is hemolytic uremic syndrome HUS. The pathogenesis of E. coli 0157:H7 infections is usually related to the ability of this bacterium to colonize on the mucosal cell and excrete its toxins and cause disease but interestingly this bacteria may colonize ruminants' gut especially cattle and cause no harm to them. The bacteria is able to survive in the human food chain due to cross contamination which is why it is a concern in public health and also a threat in veterinary field as well as food industry due to its spreading in foods. In this study 30 cattle in the Shah Alam abattoir were sampled and subjected to Immunomagnetic separation lMS technique for the isolation of E. coli 0 157:H7 to increase the sensitivity to detect the bacteria. A total of two positive samples were found. The isolates were confirmed using Polymerase Chain Reaction PCR with three specific genes comprising of the somatic 'O' and flagella 'H' antigens.

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Item Type:	Proceedings Paper
Additional Information:	1ill. 12 ref.
AGROVOC Term:	Isolation
AGROVOC Term:	Escherichia coli
AGROVOC Term:	Cattle
AGROVOC Term:	Polymerase chain reaction
Geographical Term:	MALAYSIA
Depositing User:	Ms. Norfaezah Khomsan
Last Modified:	24 Apr 2025 05:27
URI:	http://webagris.upm.edu.my/id/eprint/15829

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