In vitro callus and cell cultures of Eurycoma longifolia Jack as affected by types of explants

Chan L.K., . and Luthfi A.M. Siregar, . and Chris Teo K.H., . (2000) In vitro callus and cell cultures of Eurycoma longifolia Jack as affected by types of explants. [Proceedings Paper]

Eurycoma longifolia Jack callus could be induced from its petiole leaf and stem tissues when cultured in Murashige and Skoog MS medium containing different concentrations of naphthaleneacetic acid NAA0-10 mg/l and benzyladenine BA0-10 mg/l. Highest callus production was obtained from petiole explant when cultured in MS medium 2 mg/l NAA 2 mg/l BA. Optimum growth of callus could be induced from leaf explants when cultured in MS medium 10 mg/l NAA while stem tissues could produced the most callus in MS medium 10 mg/l NAA 2 mg/l BA. Calluses that were induced from petiole and stem tissues were yellowish green and compact in nature while the leaf explants produced loose and friable pale yellow callus. The production of callus became constant or reduced after the fifth or sixth subcultures. Cell cultures were established from the loose and friable callus induced from the leaf tissues. The cell suspension cultures were maintained in MS medium 0.5 mg/l NAA

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Abstract

Eurycoma longifolia Jack callus could be induced from its petiole leaf and stem tissues when cultured in Murashige and Skoog MS medium containing different concentrations of naphthaleneacetic acid NAA0-10 mg/l and benzyladenine BA0-10 mg/l. Highest callus production was obtained from petiole explant when cultured in MS medium 2 mg/l NAA 2 mg/l BA. Optimum growth of callus could be induced from leaf explants when cultured in MS medium 10 mg/l NAA while stem tissues could produced the most callus in MS medium 10 mg/l NAA 2 mg/l BA. Calluses that were induced from petiole and stem tissues were yellowish green and compact in nature while the leaf explants produced loose and friable pale yellow callus. The production of callus became constant or reduced after the fifth or sixth subcultures. Cell cultures were established from the loose and friable callus induced from the leaf tissues. The cell suspension cultures were maintained in MS medium 0.5 mg/l NAA

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. RS164 S471 1999 Call Number
AGROVOC Term:	SIMAROUBACEAE
AGROVOC Term:	DRUG PLANTS
AGROVOC Term:	CALLUS
AGROVOC Term:	CELL CULTURE
AGROVOC Term:	EXPLANTS
AGROVOC Term:	GROWING MEDIA
AGROVOC Term:	GROWTH
AGROVOC Term:	MEDICINAL PROPERTIES
AGROVOC Term:	MALAYSIA
Geographical Term:	MALAYSIA
Depositing User:	Ms. Norfaezah Khomsan
Last Modified:	24 Apr 2025 05:27
URI:	http://webagris.upm.edu.my/id/eprint/15892

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