A preliminary study on in vitro propagation of Eurycoma longifolia

Nor Hasnida H, . and Aziah M.Y., . and Asiah O., . and Fadhilah Z., . (2000) A preliminary study on in vitro propagation of Eurycoma longifolia. [Proceedings Paper]

Eurycoma longifolia locally known as Tongkat Ali is a traditional herb and uses of this plant for medicine vary between different places or countries. The plant takes five years or more to reach reproductive age and the fruit yield is usually low. Hence the rate of production of seeds cannot sustain the existing level of demand for this plant. Collection of this plant in the forests has become very difficult and non-economical. Commercial cultivation cannot proceed because there is no guaranteed source of high quality planting material. Therefore in vitro propagation technique is an attractive alternative to produce planting material. In our study shoot formation was induced from the shoot tips of Tongkat Ali wildings on Murashige and Skoog MS medium plus 0.1 mg/l 6 benzylaminoporine BAP and further multiplied in the same medium. But the elongation and multiplication of shoots was slow and further research is required to overcome this problem. Besides shoot formation a study for callus induction from different explants of Tongkat Ali wildings or coppices such as leaf stem petiole nodal segment and roots had been carried out. We observed that callus could be induced from these explants on MS medium plus various concentrations of hormones such as 1-naphthaleneacetic acid NAA 2 4-dichlorophenoxyacetic acid 2 4-D and combination of each with BAP.

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Abstract

Eurycoma longifolia locally known as Tongkat Ali is a traditional herb and uses of this plant for medicine vary between different places or countries. The plant takes five years or more to reach reproductive age and the fruit yield is usually low. Hence the rate of production of seeds cannot sustain the existing level of demand for this plant. Collection of this plant in the forests has become very difficult and non-economical. Commercial cultivation cannot proceed because there is no guaranteed source of high quality planting material. Therefore in vitro propagation technique is an attractive alternative to produce planting material. In our study shoot formation was induced from the shoot tips of Tongkat Ali wildings on Murashige and Skoog MS medium plus 0.1 mg/l 6 benzylaminoporine BAP and further multiplied in the same medium. But the elongation and multiplication of shoots was slow and further research is required to overcome this problem. Besides shoot formation a study for callus induction from different explants of Tongkat Ali wildings or coppices such as leaf stem petiole nodal segment and roots had been carried out. We observed that callus could be induced from these explants on MS medium plus various concentrations of hormones such as 1-naphthaleneacetic acid NAA 2 4-dichlorophenoxyacetic acid 2 4-D and combination of each with BAP.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. RS164 S471 1999 Call Number
AGROVOC Term:	DRUG PLANTS
AGROVOC Term:	HERBACEOUS PLANTS
AGROVOC Term:	SIMAROUBACEAE
AGROVOC Term:	IN VITRO EXPERIMENTATION
AGROVOC Term:	IN VITRO CULTURE
AGROVOC Term:	CULTURE MEDIA
AGROVOC Term:	CALLUS
AGROVOC Term:	MALAYSIA
Geographical Term:	MALAYSIA
Depositing User:	Ms. Norfaezah Khomsan
Last Modified:	24 Apr 2025 05:27
URI:	http://webagris.upm.edu.my/id/eprint/15899

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