Citation
Chan L.K., . and Patra R.D., . (2000) In vitro culture of Cymbopogon citratus and Cymbopogon nardus : problems and prospects. [Proceedings Paper]
Abstract
The main problem encountered in in-vitro culture of Cymbopogon species was to obtain aseptic tissue. Using a two-stage sterilisation method 70 per cent aseptic tissues with 63.3 per cent survival rate could be obtained. This was carried out by sterilising the explants with 0.01 per cent mercuric chloride for 5 minutes followed by 15 per cent CloroxR plus three drops of Tween 20 for 15 minutes rinsing with sterile distilled water three times. This was followed by a second sterilisation with 5 per cent CloroxR for 5 minutes and rinsing three times with sterile distilled water. Cymbopogon citratus was able to grow well with the formation of multiple shoots when cultured in Murashige and Skoog MS medium containing less than or equal to 6 mg/l benzyladenine BA. None of the C. citratus explants survived with the addition of more than 8 mg/l BA and 2 mg/l Indole-butyric acid IBA. When C. citratus buds cultured in MS 2 mg/l BA each explants produced an average of 21.2 shoots within 12 weeks. However abnormal shoots were produced when C. nardus was cultured in the same medium. Multiple shoot formation of C. nardus was only obtained when the explants were cultured in MS media supplemented with less than 0.5 mg/l BA and IBA.
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Abstract
The main problem encountered in in-vitro culture of Cymbopogon species was to obtain aseptic tissue. Using a two-stage sterilisation method 70 per cent aseptic tissues with 63.3 per cent survival rate could be obtained. This was carried out by sterilising the explants with 0.01 per cent mercuric chloride for 5 minutes followed by 15 per cent CloroxR plus three drops of Tween 20 for 15 minutes rinsing with sterile distilled water three times. This was followed by a second sterilisation with 5 per cent CloroxR for 5 minutes and rinsing three times with sterile distilled water. Cymbopogon citratus was able to grow well with the formation of multiple shoots when cultured in Murashige and Skoog MS medium containing less than or equal to 6 mg/l benzyladenine BA. None of the C. citratus explants survived with the addition of more than 8 mg/l BA and 2 mg/l Indole-butyric acid IBA. When C. citratus buds cultured in MS 2 mg/l BA each explants produced an average of 21.2 shoots within 12 weeks. However abnormal shoots were produced when C. nardus was cultured in the same medium. Multiple shoot formation of C. nardus was only obtained when the explants were cultured in MS media supplemented with less than 0.5 mg/l BA and IBA.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. RS164 S471 1999 Call Number |
| AGROVOC Term: | CYMBOPOGON CITRATUS |
| AGROVOC Term: | IN VITRO CULTURE |
| AGROVOC Term: | CULTURE MEDIA |
| AGROVOC Term: | STERILIZING |
| AGROVOC Term: | CHLORINATION |
| AGROVOC Term: | DISINFECTION |
| AGROVOC Term: | SHOOTS |
| AGROVOC Term: | MALAYSIA |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Norfaezah Khomsan |
| Last Modified: | 24 Apr 2025 05:27 |
| URI: | http://webagris.upm.edu.my/id/eprint/15900 |
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