Citation
Loo W.S., . and Radzali M., . and Johari R., . (2000) Natural callus flavonoid derived from stem explant of Citrus hystrix DC. Limau purut. [Proceedings Paper]
Abstract
Callus tissue culture of Citrus hystrix DC.Rutaceae was aseptically initiated from stem explant. The formation of callus derived from explants could be optimised by manipulating the plant growth regulators in a basal medium of Murashige and Skoog MS. A series of MS medium was set up with a combination of 0.20 mg/l 2 4-dichlorophenoxy acetic acid and 1.50 mg/l benzylaminopurine acid in the dark and 0.50 mg/l 2 4-dicholophenoxy acetic acid and 1.00 mg/l benzylaminopurine acid in the light. The callus formed was friable and yellowish in colour. Optimal callus growth was observed during the fourth week under dark condition and third week under light condition. Natural flavonoids hesperitin and quercetin were found in the callus tissue culture of C. hystrix.
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Abstract
Callus tissue culture of Citrus hystrix DC.Rutaceae was aseptically initiated from stem explant. The formation of callus derived from explants could be optimised by manipulating the plant growth regulators in a basal medium of Murashige and Skoog MS. A series of MS medium was set up with a combination of 0.20 mg/l 2 4-dichlorophenoxy acetic acid and 1.50 mg/l benzylaminopurine acid in the dark and 0.50 mg/l 2 4-dicholophenoxy acetic acid and 1.00 mg/l benzylaminopurine acid in the light. The callus formed was friable and yellowish in colour. Optimal callus growth was observed during the fourth week under dark condition and third week under light condition. Natural flavonoids hesperitin and quercetin were found in the callus tissue culture of C. hystrix.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. RS164 S471 1999 Call Number |
| AGROVOC Term: | CITRUS |
| AGROVOC Term: | CITRUS FRUITS |
| AGROVOC Term: | EXPLANTS |
| AGROVOC Term: | TISSUE CULTURE |
| AGROVOC Term: | CULTURE MEDIA |
| AGROVOC Term: | CALLUS |
| AGROVOC Term: | STEMS |
| AGROVOC Term: | EXTRACTION |
| AGROVOC Term: | PLANT EXTRACTS |
| AGROVOC Term: | HYDROLYSIS |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Norfaezah Khomsan |
| Last Modified: | 24 Apr 2025 05:27 |
| URI: | http://webagris.upm.edu.my/id/eprint/15903 |
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