Studies on the production of flavonoids quercetin and hesperitin from callus culture of Citrus aurantifolia Christm and Panzer swingle

Siti Mahyuni M.A., . and Radzali M., . and Marziah M., . and Johari R., . and Mohd Aspollah S., . (2000) Studies on the production of flavonoids quercetin and hesperitin from callus culture of Citrus aurantifolia Christm and Panzer swingle. [Proceedings Paper]

Callus culture derived from the peels of mature citrus aurantifolia limau nipis fruits has been successfully established in a basal medium of Murashige and Skoog MSsupplemented with 1.5 mg/L w/v alpha-naphthalene acetic acid NAA 0.5 mg/Lw/v 6-furfurylaminopurine kinetin and 3.0 g/Lw/v sucrose solidified with 3.0 g/Lw/v phytagel. Incubation of the callus cultures under dark and light conditions showed similar sigmoid pattern in which the maximum growth for both was obtained in the 6th week of incubation. The levels of the major flavonoids quercetin and hesperetin were measured by HPLC. Callus cultures incubated in the light and dark conditions were found to contain respectively 19.73 and 1.90 microgram quercetin per gram of dry tissue weight. The hesperitin content in callus cultures incubated under light and dark conditions were only 0.64 and 0.58 microgram/gram of dry tissue weight respectively. Supplementation with relatively low concentrations of yeast extract YEi.e 0.5 1.0 and 2.0g/Lw/v to the culture medium did not affect the quercetin and hesperitin levels but supplementation with higher concertrations of YE up to 4.0 g/Lw/v was found to decrease the levels of quercetin and hesperitin by 18.9 per cent and 7.8 per cent respectively. Supplementation with high concentrations of YE also caused browning and decreased growth and cell viability under both dark and light conditions.

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Abstract

Callus culture derived from the peels of mature citrus aurantifolia limau nipis fruits has been successfully established in a basal medium of Murashige and Skoog MSsupplemented with 1.5 mg/L w/v alpha-naphthalene acetic acid NAA 0.5 mg/Lw/v 6-furfurylaminopurine kinetin and 3.0 g/Lw/v sucrose solidified with 3.0 g/Lw/v phytagel. Incubation of the callus cultures under dark and light conditions showed similar sigmoid pattern in which the maximum growth for both was obtained in the 6th week of incubation. The levels of the major flavonoids quercetin and hesperetin were measured by HPLC. Callus cultures incubated in the light and dark conditions were found to contain respectively 19.73 and 1.90 microgram quercetin per gram of dry tissue weight. The hesperitin content in callus cultures incubated under light and dark conditions were only 0.64 and 0.58 microgram/gram of dry tissue weight respectively. Supplementation with relatively low concentrations of yeast extract YEi.e 0.5 1.0 and 2.0g/Lw/v to the culture medium did not affect the quercetin and hesperitin levels but supplementation with higher concertrations of YE up to 4.0 g/Lw/v was found to decrease the levels of quercetin and hesperitin by 18.9 per cent and 7.8 per cent respectively. Supplementation with high concentrations of YE also caused browning and decreased growth and cell viability under both dark and light conditions.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. RS164 S471 1999 Call Number
AGROVOC Term:	CITRUS AURANTIIFOLIA
AGROVOC Term:	CALLUS
AGROVOC Term:	TISSUE CULTURE
AGROVOC Term:	QUERCETIN
AGROVOC Term:	MALAYSIA
Geographical Term:	MALAYSIA
Depositing User:	Ms. Norfaezah Khomsan
Last Modified:	24 Apr 2025 05:27
URI:	http://webagris.upm.edu.my/id/eprint/15904

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