Search for repellent active compound from hexane extract of nonvolatile fraction of Citrus nobilis peel with mosquito Aedes aegypti as bioindicator

Euis Julaeha, . and Dewi Nuraeni, . and Kurnia D., . and Roekmi-Ati T.J., . (2001) Search for repellent active compound from hexane extract of nonvolatile fraction of Citrus nobilis peel with mosquito Aedes aegypti as bioindicator. [Proceedings Paper]

Search for repellent active compound from hexane extract of nonvolatile fraction of Citrus nobilis peel had been done using mosquito Aedes aegypti as bio indicator. The C. nobilis peels were steam distillated to separate volatile fraction and the residue followed through extraction with hexane to prepare nonvolatile fraction. In a dose 10 000 ppm the activity of extract to refuse against mosquito A. aegypti was 94.54. The hexane exract was separated on silica gel G60 column chromatography with solvent gradient : hexane dichloromethane ethyl acetate methanol respectively. The result 8 fractions were obtained and bioassayed against Aedes aegypti. The fraction F Rf0.23 m0.38 g A92.10 had highest in activity. Its separation on silica gel G60 with solvents : hexane : dichloromethane : ethyl acetate1 : 4: 0.5 yield 4 fractions where the fraction F1 Rf0.76 m0.009 g A94.82 gave highest activity. The fraction F1 was purified on preparative thin layer chromatography precoated silica gel F254 and hexane : dichloromethane3 : 2 as solvent. From this 2 fraction were obtained that were fractions FA1 Rf0.49 m0.001 g A85.71 and FA2 Rf0.30 m0.0008 g A80. The result of phytochemistry testing showed triterpene group compound were exist in both fraction FA1 and FA2 and their GC-MS analysis gave 5 and 4 major peaks respectively.

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Abstract

Search for repellent active compound from hexane extract of nonvolatile fraction of Citrus nobilis peel had been done using mosquito Aedes aegypti as bio indicator. The C. nobilis peels were steam distillated to separate volatile fraction and the residue followed through extraction with hexane to prepare nonvolatile fraction. In a dose 10 000 ppm the activity of extract to refuse against mosquito A. aegypti was 94.54. The hexane exract was separated on silica gel G60 column chromatography with solvent gradient : hexane dichloromethane ethyl acetate methanol respectively. The result 8 fractions were obtained and bioassayed against Aedes aegypti. The fraction F Rf0.23 m0.38 g A92.10 had highest in activity. Its separation on silica gel G60 with solvents : hexane : dichloromethane : ethyl acetate1 : 4: 0.5 yield 4 fractions where the fraction F1 Rf0.76 m0.009 g A94.82 gave highest activity. The fraction F1 was purified on preparative thin layer chromatography precoated silica gel F254 and hexane : dichloromethane3 : 2 as solvent. From this 2 fraction were obtained that were fractions FA1 Rf0.49 m0.001 g A85.71 and FA2 Rf0.30 m0.0008 g A80. The result of phytochemistry testing showed triterpene group compound were exist in both fraction FA1 and FA2 and their GC-MS analysis gave 5 and 4 major peaks respectively.

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Item Type:	Proceedings Paper
Additional Information:	Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QD415 A1N277 2000
AGROVOC Term:	CITRUS
AGROVOC Term:	CULICIDAE
AGROVOC Term:	REPELLENTS
AGROVOC Term:	EXTRACTS
AGROVOC Term:	INDONESIA
Geographical Term:	MALAYSIA
Depositing User:	Ms. Norfaezah Khomsan
Last Modified:	24 Apr 2025 05:27
URI:	http://webagris.upm.edu.my/id/eprint/15914

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